PMID: 10144Sep 1, 1976

Preservation of various microsomal drug metabolizing components in tissue preparations from the livers, lungs, and small intestines of rodents

Drug Metabolism and Disposition : the Biological Fate of Chemicals
J M Tredger, R S Chhabra


The livers, lungs, and small intestines of untreated rabbits and the livers of control rats were stored intact, or as microsomal suspensions, under liquid nitrogen at -196 degrees C. Aniline hydroxylase, aminopyrine demethylase, benzpyrene hydroxylase, biphenyl hydroxylase, NADPH-cytochrome c reductase, UDP-glucuronyltransferase activities, the microsomal content of cytochrome P-450, and the aniline- and benzphetamine-induced spectral changes were compared in fresh and stored preparations. Few significant changes in any of the above parameters resulted from storage of rabbit tissue preparations in liquid nitrogen for periods of up to 28 days. Pretreatment of rabbits with phenobarbital did not affect the stability of their stored microsomal preparations. Enzyme activities in the livers of untreated or 3-methylcholanthrene-pretreated rats were less stable to storage than in tissue preparations from rabbits stored under identical conditions. However, when rat hepatic microsomes were resuspended in KCl-HEPES supplemented with 1 mM EDTA before storage, enzyme activities were largely unaffected by freezing in liquid nitrogen.

Related Concepts

Aminopyrine N-Demethylase
Aniline Hydroxylase
Cytochrome P-450 Oxygenase
Intestines, Small
Microsomes, Liver
NADPH-Ferrihemoprotein Reductase

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

The Tendon Seed Network

Tendons are rich in the extracellular matrix and are abundant throughout the body providing essential roles including structure and mobility. The transcriptome of tendons is being compiled to understand the micro-anatomical functioning of tendons. Discover the latest research pertaining to the Tendon Seed Network here.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.


Incretins are metabolic hormones that stimulate a decrease in glucose levels in the blood and they have been implicated in glycemic regulation in the remission phase of type 1 diabetes. Here is the latest research.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Long COVID-19

“Long Covid-19” describes illness in patients who are reporting long-lasting effects of the SARS-CoV-19 infection, often long after they have recovered from acute Covid-19. Ongoing health issues often reported include low exercise tolerance and breathing difficulties, chronic tiredness, and mental health problems such as post-traumatic stress disorder and depression. This feed follows the latest research into Long Covid.

Spatio-Temporal Regulation of DNA Repair

DNA repair is a complex process regulated by several different classes of enzymes, including ligases, endonucleases, and polymerases. This feed focuses on the spatial and temporal regulation that accompanies DNA damage signaling and repair enzymes and processes.

© 2021 Meta ULC. All rights reserved