Prespore-to-stalk conversion involves the production of a pathway-specific glycoprotein, wst25, in the cellular slime mould Dictyostelium discoideum

Biochemical and Biophysical Research Communications
Y KuboharaK Okamoto

Abstract

We have previously identified a stalk-specific wheat germ agglutinin (WGA)-binding protein, wst34, in the cellular slime mould Dictyostelium discoideum [Biochem. Cell Biol. 68 (1990) 699]. Here, we found another stalk-specific WGA-binding protein, wst25, which was detected with two antisera that recognize wst34. Using the two marker proteins, we then analyzed and compared the pathways of prestalk-to-stalk maturation and prespore-to-stalk conversion in vitro and in vivo. Prestalk cells isolated from normally formed slugs can be converted to stalk cells (designated StI) in vitro with 8-bromo-cAMP (Br-cAMP), whereas prespore cells isolated from slugs can be converted to fully vacuolated stalk cells (designated StII) in vitro with Br-cAMP and DIF-1. During the process of prespore-to-stalk conversion, prespore-specific mRNAs, D19 and 2H3, disappeared rapidly, while prestalk-specific mRNAs, ecmA and ecmB, appeared at 2h of incubation and increased thereafter. Most importantly, however, the StII cells thus formed were biochemically different from the StI cells originated from prestalk cells; that is, StI cells expressed wst34 but not wst25, while StII cells expressed wst25 but not wst34. When prespore cells isolated from slugs were al...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K MannR A Firtel
Oct 1, 1985·Analytical Biochemistry·P K SmithD C Klenk
Jan 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·J T Bonner
Jul 1, 1993·Experimental Cell Research·Y KuboharaK Okamoto
Feb 1, 1993·Experimental Cell Research·Y Kubohara, K Okamoto

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