Pressure dependence of amide hydrogen-deuterium exchange rates for individual sites in T4 lysozyme

Biochemistry
T K Hitchens, Robert G Bryant

Abstract

We report measurements of the pressure dependence of rate constants for the exchange of amide residue protons with solvent deuterium for T4 lysozyme. Data obtained at nine pressures from 0.1 to 200 MPa are analyzed using an elementary kinetic model and the formalism of transition state theory which yield activation volumes for the exchange process. Resolution of individual amide sites was accomplished using the HSQC two-dimensional (2D) NMR experiment on uniformly (15)N-labeled protein. The observed activation volumes span the range from 2.75 to -25.1 mL/mol at 22 degreesC and pH* 7.5. When corrected for the pressure dependence of the ionic product for water and for the reported activation volume for the amide exchange reaction in model compounds, the portion of the activation volume associated with the accessibility of the solvent or catalyst to the amide sites ranges from -15.1 to 12.8 mL/mol. There is no simple correlation between the activation volumes and the protection factors for amide hydrogen exchange. The activation volumes for residues in close proximity in either the primary sequence or the folded structure may differ considerably. There is no trivial correlation between the activation volume and the secondary struc...Continue Reading

References

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Citations

May 2, 2002·Biochimica Et Biophysica Acta·Patrizia Cioni, Giovanni B Strambini
May 2, 2002·Biochimica Et Biophysica Acta·Boonchai B BoonyaratanakornkitDouglas S Clark
Nov 5, 1999·Protein Science : a Publication of the Protein Society·K AkasakaC K Woodward
May 4, 2000·Protein Science : a Publication of the Protein Society·H R KalbitzerK Akasaka
Feb 13, 2007·Journal of Molecular Biology·Marcus D CollinsSol M Gruner
Jan 8, 2011·Protein Science : a Publication of the Protein Society·Nicholas C FitzkeeAd Bax
Mar 3, 2004·Biophysical Journal·Ching-Ling Teng, Robert G Bryant
Jun 27, 2000·Biophysical Journal·D P Kharakoz
Feb 25, 2015·Quarterly Reviews of Biophysics·Nicholas J Anthis, G Marius Clore
May 11, 2006·Chemical Reviews·Kazuyuki Akasaka

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