PMID: 6432533Aug 1, 1984

Pressure dependence of thermolysin catalysis

European Journal of Biochemistry
M Fukuda, S Kunugi


A comparison of the pressure and temperature dependences of the catalytic reaction of thermolysin, a thermostable neutral protease from Bacillus thermoproteolyticus, with those of a non-thermostable neutral protease from Bacillus subtilis revealed a distinct difference in Km values of these enzymes for 3-(2-furyl)acrylyl-blocked dipeptide and tripeptide substrates, but not for Kcat. Namely, the volume changes for the binding process (delta V) for these substrates and several competitive inhibitors were -20- -30 ml/mol for thermolysin and nearly 0 ml/mol for the non-thermostable neutral protease. The enthalpy and entropy changes for the binding process were negative for thermolysin, but positive for the latter enzyme. The activation volumes (delta V not equal to) for the kcat process were 25 -35 ml/mol for both proteases, and activation enthalpy and entropy showed no significant difference between the two enzymes. The characteristic difference in the pressure and the temperature dependences seen for the binding process is discussed in relation to the thermostability of the proteases.


Jan 13, 1976·Biochemistry·B Holmquist, B L Vallee
Apr 1, 1977·Physiological Reviews·M J Peach
Jan 1, 1978·CRC Critical Reviews in Biochemistry·R S Roche, G Voordouw
Dec 11, 1979·Biochemistry·P ArgosJ D Tratschin
Aug 22, 1978·Biochemistry·G Morgan, J S Fruton
Nov 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P L LevyK A Walsh
Aug 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·P S Low, G N Somero
May 22, 1976·Journal of the American Chemical Society·R C NeumanG D Lockyer
Oct 1, 1972·Archives of Biochemistry and Biophysics·R K Williams, C Shen
Mar 12, 1974·Biochemistry·J FederB S Wildi
Aug 1, 1970·European Journal of Biochemistry·K Morihara, H Tsuzuki
Oct 14, 1972·Journal of Molecular Biology·P M ColmanB W Matthews
Oct 8, 1969·Biochemical and Biophysical Research Communications·S A LattB L Vallee
Jun 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S I Wayne, J S Fruton
Jan 1, 1981·Annual Review of Biophysics and Bioengineering·R Jaenicke
Jan 1, 1982·Progress in Biophysics and Molecular Biology·G R WelchS Damjanovich
Jan 1, 1982·Annual Review of Biophysics and Bioengineering·K Heremans
May 21, 1982·Biochimica Et Biophysica Acta·S KunugiN Ise
Sep 1, 1980·Journal of Biochemistry·T Oka, K Morihara


Sep 5, 1993·Biotechnology and Bioengineering·J Kim, J S Dordick
Mar 1, 1994·Journal of Molecular Recognition : JMR·H KabataS Kunugi
May 2, 2002·Biochimica Et Biophysica Acta·S Kunugi, Naoki Tanaka
Sep 22, 1999·European Journal of Biochemistry·K RuanC Balny
Jun 18, 1985·European Journal of Biochemistry·M Fukuda, S Kunugi
Oct 15, 1996·European Journal of Biochemistry·S KunugiK Oda
Nov 5, 1997·European Journal of Biochemistry·S KunugiC Balny
Jan 1, 1990·Annals of the New York Academy of Sciences·S Kunugi, A Nomura
Nov 30, 1992·Annals of the New York Academy of Sciences·S Kunugi
Oct 6, 2009·Applied Biochemistry and Biotechnology·Nattascha KyawMaria Alice Zarur Coelho
Jan 1, 1991·Critical Reviews in Biotechnology·J M Ludlow, D S Clark
May 5, 1999·FEMS Microbiology Letters·A G Macdonald, B Martinac
Sep 1, 1996·International Journal of Peptide and Protein Research·N TanakaS Kunugi

Related Concepts

Natto Bacteria
Hydrogen-Ion Concentration
Hydrostatic Pressure

Trending Feeds


Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Synthetic Genetic Array Analysis

Synthetic genetic arrays allow the systematic examination of genetic interactions. Here is the latest research focusing on synthetic genetic arrays and their analyses.

Congenital Hyperinsulinism

Congenital hyperinsulinism is caused by genetic mutations resulting in excess insulin secretion from beta cells of the pancreas. Here is the latest research.

Neural Activity: Imaging

Imaging of neural activity in vivo has developed rapidly recently with the advancement of fluorescence microscopy, including new applications using miniaturized microscopes (miniscopes). This feed follows the progress in this growing field.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Epigenetic Memory

Epigenetic memory refers to the heritable genetic changes that are not explained by the DNA sequence. Find the latest research on epigenetic memory here.

Cell Atlas of the Human Eye

Constructing a cell atlas of the human eye will require transcriptomic and histologic analysis over the lifespan. This understanding will aid in the study of development and disease. Find the latest research pertaining to the Cell Atlas of the Human Eye here.

Femoral Neoplasms

Femoral Neoplasms are bone tumors that arise in the femur. Discover the latest research on femoral neoplasms here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.