PMID: 6432533Aug 1, 1984

Pressure dependence of thermolysin catalysis

European Journal of Biochemistry
M Fukuda, S Kunugi

Abstract

A comparison of the pressure and temperature dependences of the catalytic reaction of thermolysin, a thermostable neutral protease from Bacillus thermoproteolyticus, with those of a non-thermostable neutral protease from Bacillus subtilis revealed a distinct difference in Km values of these enzymes for 3-(2-furyl)acrylyl-blocked dipeptide and tripeptide substrates, but not for Kcat. Namely, the volume changes for the binding process (delta V) for these substrates and several competitive inhibitors were -20- -30 ml/mol for thermolysin and nearly 0 ml/mol for the non-thermostable neutral protease. The enthalpy and entropy changes for the binding process were negative for thermolysin, but positive for the latter enzyme. The activation volumes (delta V not equal to) for the kcat process were 25 -35 ml/mol for both proteases, and activation enthalpy and entropy showed no significant difference between the two enzymes. The characteristic difference in the pressure and the temperature dependences seen for the binding process is discussed in relation to the thermostability of the proteases.

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Citations

Sep 5, 1993·Biotechnology and Bioengineering·J Kim, J S Dordick
Mar 1, 1994·Journal of Molecular Recognition : JMR·H KabataS Kunugi
May 2, 2002·Biochimica Et Biophysica Acta·S Kunugi, Naoki Tanaka
Sep 22, 1999·European Journal of Biochemistry·K RuanC Balny
Jun 18, 1985·European Journal of Biochemistry·M Fukuda, S Kunugi
Oct 15, 1996·European Journal of Biochemistry·S KunugiK Oda
Nov 5, 1997·European Journal of Biochemistry·S KunugiC Balny
Jan 1, 1990·Annals of the New York Academy of Sciences·S Kunugi, A Nomura
Nov 30, 1992·Annals of the New York Academy of Sciences·S Kunugi
Oct 6, 2009·Applied Biochemistry and Biotechnology·Nattascha KyawMaria Alice Zarur Coelho
Jan 1, 1991·Critical Reviews in Biotechnology·J M Ludlow, D S Clark
May 5, 1999·FEMS Microbiology Letters·A G Macdonald, B Martinac
Sep 1, 1996·International Journal of Peptide and Protein Research·N TanakaS Kunugi

Related Concepts

Natto Bacteria
Catalysis
Hydrogen-Ion Concentration
Hydrostatic Pressure
Thermodynamics
Thermolysin

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