Pressure effects on the lateral distribution of cholesterol in lipid bilayers: a time-resolved spectroscopy study

Biophysical Journal
P TaucJ C Brochon

Abstract

The effects of hydrostatic pressure and temperature on the phase behavior and physical properties of the binary mixture palmitoyloleoylphosphatidylcholine/cholesterol, over the 0-40 molar % range of cholesterol compositions, were determined from the changes in the fluorescence lifetime distribution and anisotropy decay parameters of the natural lipid trans-parinaric acid (t-PnA). Pressurized samples were excited with a Ti-sapphire subpicosecond laser, and fluorescence decays were analyzed by the quantified maximum entropy method. Above the transition temperature (T(T) = -5 degrees C), at atmospheric pressure, two liquid-crystalline phases, alpha and beta, are formed in this system. At each temperature and cholesterol concentration below the transition pressure, the fluorescence lifetime distribution pattern of t-PnA was clearly modulated by the pressure changes. Pressure increased the fraction of the liquid-ordered beta-phase and its order parameter, but it decreased the amount of cholesterol in this phase. Palmitoyloleoylphosphatidylcholine/cholesterol phase diagrams were also determined as a function of temperature and hydrostatic pressure.

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Citations

Jun 6, 2002·Chemistry and Physics of Lipids·Rumiana Koynova, Martin Caffrey
Sep 29, 1999·Bioscience, Biotechnology, and Biochemistry·M Kato, R Hayashi
Jul 4, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Marina PinheiroSalette Reis
Nov 13, 2004·Journal of the American Chemical Society·A Salomé VeigaMiguel A R B Castanho

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