Pressure effects on the structure of oligomeric proteins prior to subunit dissociation

Journal of Molecular Biology
Patrizia Cioni, G B Strambini

Abstract

In studies of pressure-induced subunit dissociation of protein aggregates, now widely used to evaluate the association free energy, entropy and enthalpy of very stable complexes, it is assumed that high pressure does not influence their structure/thermodynamic parameters and that some peculiarities of these equilibria, such as the decrease in subunit affinity at larger degrees of dissociation (alpha) and hysteresis in alpha/pressure diagrams are imputable to the slow conformational drift of isolated subunits. To test this premise, the conformation of dimeric alcohol dehydrogenase from horse liver and alkaline phosphatase from Escherichia coli was monitored as a function of pressure (up to 3 kbar) and temperature (0 to 50 degrees C) by means of the intrinsic Trp fluorescence and phosphorescence emission and binding of the 1-anilinonaphatalene-8-sulphonic acid (ANS) fluorophore. The results show a distinct influence of high pressure on the native dimers whose changes in conformation may, depending on whether or not these alterations are promptly reversed, be distinguished in elastic and inelastic changes. Elastic changes are ubiquitous and refer to pronounced modulations of the phosphorescence lifetime which is a monitor of the i...Continue Reading

Citations

Nov 5, 1999·Protein Science : a Publication of the Protein Society·K AkasakaC K Woodward
May 25, 1999·Protein Science : a Publication of the Protein Society·M M SunD S Clark
Mar 21, 1998·Proceedings of the National Academy of Sciences of the United States of America·G HummerL R Pratt
Oct 25, 2006·The FEBS Journal·Eleonora NicolaiGiampiero Mei
Sep 20, 2016·Biotechnology and Bioengineering·Ali HalalipourJosé I Reyes-De-Corcuera
Sep 18, 2004·European Journal of Biochemistry·M Judith KornblattClaude Balny
Jan 11, 2003·Biotechnology & Genetic Engineering Reviews·Antonio D Molina-García
Feb 27, 2018·Physical Chemistry Chemical Physics : PCCP·Karin JuliusRoland Winter
Dec 21, 2002·European Journal of Biochemistry·Marie TrovasletMarie Dominique Legoy
May 31, 2017·Enzyme and Microbial Technology·Ali HalalipourJosé I Reyes-De-Corcuera
Dec 1, 2020·Journal of the American Chemical Society·Kristian Le VayAdam W Perriman
Jun 21, 2001·Archives of Biochemistry and Biophysics·P CioniG B Strambini
May 2, 2002·Biochimica Et Biophysica Acta·Patrizia Cioni, Giovanni B Strambini
May 2, 2002·Biochimica Et Biophysica Acta·Boonchai B BoonyaratanakornkitDouglas S Clark
Sep 12, 1998·Biochimica Et Biophysica Acta·K Heremans, L Smeller
May 15, 2002·Biochimica Et Biophysica Acta·Naoki TanakaShigeru Kunugi
Sep 26, 2002·International Journal of Biological Macromolecules·Tomohito Iwasaki, Kastuhiro Yamamoto
Mar 29, 2005·Biophysical Journal·Patrizia CioniGiovanni B Strambini
Oct 16, 2002·Biochemistry·Markandeswar PandaPaul M Horowitz

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