PMID: 11322896Apr 27, 2001Paper

Pressure-exploration of the 33-kDa protein from the spinach photosystem II particle

European Journal of Biochemistry
K RuanC Balny

Abstract

The 33-kDa protein isolated from the spinach photosystem II particle is an ideal model to explore high-pressure protein-unfolding. The protein has a very low free energy as previously reported by chemical unfolding studies, suggesting that it must be easy to modulate its unfolding transition by rather mild pressure. Moreover, the protein molecule consists of only one tryptophan residue (Trp241) and eight tyrosine residues, which can be conveniently used to probe the protein conformation and structural changes under pressure using either fluorescence spectroscopy or fourth derivative UV absorbance spectroscopy. The different experimental methods used in the present study indicate that at 20 degrees C and pH 6, the 33-kDa protein shows a reversible two-state unfolding transition from atmospheric pressure to about 180 MPa. This value is much lower than those found for the unfolding of most proteins studied so far. The unfolding transition induces a large red shift of the maximum fluorescence emission of 34 nm (from 316 nm to 350 nm). The change in standard free energy (DeltaGo) and in volume (DeltaV) for the transition at pH 6.0 and 20 degrees C are -14.6 kJ.mol-1 and -120 mL.mol-1, respectively, in which the DeltaGo value is cons...Continue Reading

References

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Citations

May 2, 2002·Biochimica Et Biophysica Acta·László Smeller
May 2, 2002·Biochimica Et Biophysica Acta·Reinhard Lange, Claude Balny
May 2, 2002·Biochimica Et Biophysica Acta·Kangcheng Ruan, Claude Balny
Apr 16, 2003·European Journal of Biochemistry·Kangcheng RuanClaude Balny
Dec 29, 2004·Biophysical Chemistry·Paul E Smith
Feb 17, 2006·Biochimica Et Biophysica Acta·Joan TorrentReinhard Lange
Oct 25, 2016·Journal of Theoretical Biology·Marek IngrReinhard Lange
Aug 6, 2005·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C A Royer
Feb 1, 2006·Biochimica Et Biophysica Acta·Cui-Yan TanKang-Cheng Ruan
Feb 5, 2003·Biochemistry·Joan TorrentReinhard Lange
May 2, 2002·Biochimica Et Biophysica Acta·Catherine A Royer
Jun 11, 2002·Biochemical and Biophysical Research Communications·Kangcheng RuanClaude Balny
Sep 25, 2009·Biochemistry·Szabolcs OsváthLászló Smeller

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