'Pressure-flow'-triggered intracellular Ca2+ transients in rat cardiac myocytes: possible mechanisms and role of mitochondria

The Journal of Physiology
Stephen Belmonte, M Morad

Abstract

Cardiac myocytes, in the intact heart, are exposed to shear/fluid forces during each cardiac cycle. Here we describe a novel Ca(2+) signalling pathway, generated by 'pressurized flows' (PFs) of solutions, resulting in the activation of slowly developing ( approximately 300 ms) Ca(2+) transients lasting approximately 1700 ms at room temperature. Though subsequent PFs (applied some 10-30 s later) produced much smaller or undetectable responses, such transients could be reactivated following caffeine- or KCl-induced Ca(2+) releases, suggesting that a small, but replenishable, Ca(2+) pool serves as the source for their activation. PF-triggered Ca(2+) transients could be activated in Ca(2+)-free solutions or in solutions that block voltage-gated Ca(2+) channels, stretch-activated channels (SACs), or the Na(+)-Ca(2+) exchanger (NCX), using Cd(2+), Gd(3+), or Ni(2+), respectively. PF-triggered Ca(2+) transients were significantly smaller in quiescent than in electrically paced myocytes. Paced Ca(2+) transients activated at the peak of PF-triggered Ca(2+) transients were not significantly smaller than those produced normally, suggesting functionally separate Ca(2+) pools for paced and PF-triggered transients. Suppression of nitric oxid...Continue Reading

References

Oct 1, 1991·The American Journal of Physiology·H MiyataR G Hansford
Nov 1, 1985·The American Journal of Physiology·R Mitra, M Morad
Apr 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·P F DaviesM A Gimbrone
Jun 1, 1997·Journal of Molecular and Cellular Cardiology·H Hu, F Sachs
Sep 2, 1998·Proceedings of the National Academy of Sciences of the United States of America·L CleemannM Morad
Oct 3, 1999·Annals of Internal Medicine·M D Ezekowitz
Feb 26, 2000·Cardiovascular Research·I Fleming, R Busse
Mar 16, 2000·The Journal of Physiology·Y H ZhangY E Earm
May 16, 2000·The Journal of Biological Chemistry·G SzalaiG Hajnóczky
Nov 25, 2000·Circulation Research·M LöhnM Gollasch
Apr 12, 2001·The Journal of Biological Chemistry·G BeutnerS S Sheu
Oct 16, 2001·The Journal of Physiology·J A SánchezS S Sheu
Feb 21, 2002·Proceedings of the National Academy of Sciences of the United States of America·Pál PacherGyörgy Hajnóczky
May 30, 2002·The Journal of General Physiology·Guangju JiMichael I Kotlikoff
Jan 30, 2003·Journal of Cardiac Failure·Thomas ForceSyed Haq
May 7, 2003·Progress in Biophysics and Molecular Biology·S C CalaghanE White
May 7, 2003·Progress in Biophysics and Molecular Biology·Tara L Riemer, Leslie Tung
May 7, 2003·Progress in Biophysics and Molecular Biology·Weizhen Niu, Frederick Sachs
Jun 21, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Victor RizzoPeter F Davies
Aug 23, 2003·Circulation Research·James N WeissPeipei Ping
Apr 20, 2004·American Journal of Physiology. Lung Cellular and Molecular Physiology·Mir H AliPaul T Schumacker
Jul 31, 2004·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Allen KaasikVladimir Veksler
Aug 11, 2004·Molecular Aspects of Medicine·Michael R Duchen
Oct 9, 2004·American Journal of Physiology. Heart and Circulatory Physiology·C Radel, V Rizzo
Nov 26, 2004·American Journal of Physiology. Heart and Circulatory Physiology·Masao SaotomeHideharu Hayashi
Feb 1, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Zuojun Ren, Clive M Baumgarten
Jul 5, 2005·Cardiovascular Research·John R Dietz
Aug 12, 2005·Annals of the New York Academy of Sciences·Martin MoradSteve Belmonte
May 26, 2006·American Journal of Physiology. Cell Physiology·Marina SedovaLothar A Blatter
Sep 19, 2006·Cardiovascular Research·Jonathan P BrennanMichael J Shattock

❮ Previous
Next ❯

Citations

Jul 14, 2010·Cardiovascular Research·David A Brown, Brian O'Rourke
Mar 5, 2013·Cardiovascular Research·Huiliang ZhangHeping Cheng
Dec 18, 2012·Biochemistry. Biokhimii︠a︡·V I Kapelko
Jan 30, 2013·Cell Calcium·Angelo O RosaMartin Morad
Aug 19, 2011·Progress in Biophysics and Molecular Biology·D D ChanC P Neu
May 7, 2015·Biomicrofluidics·Yu-Fang HsiaoChau-Hwang Lee
Feb 19, 2017·Biochimica Et Biophysica Acta. Molecular Cell Research·Joon-Chul KimSun-Hee Woo
Mar 25, 2017·Nature Communications·Chang Seok LeeSusan L Hamilton
Jun 27, 2017·Progress in Biophysics and Molecular Biology·Patrick SchönleitnerGudrun Antoons
Jul 13, 2017·International Journal of Molecular Sciences·Aderville Cabassi, Michele Miragoli
Mar 19, 2014·British Journal of Pharmacology·E Fernández-SadaG García-Rivas
Oct 10, 2014·British Journal of Pharmacology·O PechánováP Ferdinandy
Dec 4, 2019·The Journal of Physiology·Leighton T IzuYe Chen-Izu
Dec 25, 2016·Europace : European Pacing, Arrhythmias, and Cardiac Electrophysiology : Journal of the Working Groups on Cardiac Pacing, Arrhythmias, and Cardiac Cellular Electrophysiology of the European Society of Cardiology·T Alexander Quinn, Peter Kohl
Oct 12, 2018·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·E A Rog-ZielinskaP Kohl
Jan 8, 2016·American Journal of Physiology. Cell Physiology·Christopher G ScheitlinB Rita Alevriadou
Jul 14, 2017·Archives of Pharmacal Research·Joon-Chul KimSun-Hee Woo
Jan 27, 2019·Archives of Biochemistry and Biophysics·Jessica L CaoJin O-Uchi
Aug 16, 2014·Progress in Biophysics and Molecular Biology·Ken WangChristian Bollensdorff
Dec 23, 2008·Biochimica Et Biophysica Acta·Tullio Pozzan, Rüdiger Rudolf
Jan 24, 2009·Journal of Molecular and Cellular Cardiology·Brian O'Rourke, Lothar A Blatter
Feb 28, 2013·Biophysical Journal·Asuka HatanoSeiryo Sugiura

❮ Previous
Next ❯

Related Concepts

Related Feeds

Calcium & Bioenergetics

Bioenergetic processes, including cellular respiration and photosynthesis, concern the transformation of energy by cells. Here is the latest research on the role of calcium in bioenergetics.