Pressure-loaded MSCs during early osteodifferentiation promote osteoclastogenesis by increase of RANKL/OPG ratio

Annals of Biomedical Engineering
Jun LiuJun Wang

Abstract

Mechanical stress plays an important role in bone remodeling. However, it is still unclear whether mechanical stress regulates osteoclastogenesis mediated by mesenchymal stem cells (MSCs) during initial osteodifferentiation. We investigated the effects of static and dynamic pressures on osteoclast-inducing potential of MSCs during early osteodifferentiation. The osteoclastogenesis was examined using TRAP staining. The mRNA levels of receptor activator of nuclear factor-kappaB ligand (RANKL) and osteoprotegerin (OPG) genes were analyzed using real-time RT-PCR. It was shown that MSCs exposed to either pressure during initial osteodifferentiation promoted osteoclastogenesis with the up-regulation of RANKL/OPG ratio. MSCs displayed diverse responses to pressures at different points of initial osteodifferentiation. The RANKL/OPG ratio was significantly increased after osteoinduction in the primary MSCs without pressures exposure, which contradicted the previous report. These results suggest novel mechanisms of the initial biological responses of bone remodeling upon mechanical stimuli.

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Citations

Oct 13, 2009·Critical Reviews in Eukaryotic Gene Expression·Alexander G Robling, Charles H Turner
Aug 13, 2011·Journal of Orthopaedic Science : Official Journal of the Japanese Orthopaedic Association·Wei Wei HouHai Yang Yu
Feb 24, 2011·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·Esther LauLidan You
Mar 5, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Ronald Y KwonJohn A Frangos
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Aug 20, 2010·Acta Orthopaedica·Anna FahlgrenPer Aspenberg
Oct 26, 2016·Molecular Medicine Reports·Masaaki IkedaJunichiro Iida

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