Pressure-mediated vasoconstriction of juxtamedullary afferent arterioles involves P2-purinoceptor activation

The American Journal of Physiology
Edward W InschoL Gabriel Navar

Abstract

This study was conducted to examine the hypothesis that P2 purinoceptors contribute to pressure-induced autoregulatory adjustments of afferent arteriolar caliber. Experiments were performed in vitro using the blood-perfused juxtamedullary nephron technique. Afferent arteriolar diameter averaged 19.2 +/- 0.6 microns (n = 51) at control perfusion pressure of 100 mmHg and decreased when perfusion pressure was increased. Desensitization of P2 purinoceptors abolished the alpha, beta-methylene ATP-mediated afferent vasoconstriction and prevented pressure-dependent autoregulatory adjustments in afferent diameter. P2-purinoceptor saturation significantly decreased afferent caliber and attenuated pressure-induced autoregulatory responses. To block P2 receptors, afferent arterioles were treated with the P2-purinoceptor antagonists, pyridoxal-phosphate-6-azophenyl-2',4'-disulfonic acid or suramin. P2-receptor blockade prevented the afferent arteriolar vasoconstriction evoked by increasing perfusion pressure from 100 to 130 and 160 mmHg. These data demonstrate that inhibition of P2 purinoceptor-dependent responses through receptor desensitization, receptor saturation, or purinoceptor blockade impairs normal autoregulatory behavior in rat j...Continue Reading

Citations

Jun 19, 2002·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Akira Nishiyama, L Gabriel Navar
Sep 5, 2002·Annual Review of Physiology·Jürgen Schnermann, David Z Levine
Aug 19, 2004·The Journal of Pharmacology and Experimental Therapeutics·Xueying ZhaoJohn D Imig
Oct 19, 2005·British Journal of Pharmacology·Edward W InschoDavid M Pollock
Oct 11, 2005·American Journal of Physiology. Heart and Circulatory Physiology·Akira NishiyamaL Gabriel Navar
Jan 11, 2007·American Journal of Physiology. Renal Physiology·Ian RiveraEdward W Inscho
Apr 16, 2010·Physiological Reviews·Hayo CastropCharlotte Wagner
Jan 15, 2002·American Journal of Physiology. Renal Physiology·Edward W Inscho, Anthony K Cook
Oct 23, 2001·American Journal of Physiology. Heart and Circulatory Physiology·X ZhaoJ D Imig
May 16, 2001·American Journal of Physiology. Renal Physiology·E W Inscho
Jan 14, 2003·Annual Review of Physiology·P Darwin BellJános Peti-Peterdi
Jan 13, 2005·American Journal of Physiology. Renal Physiology·Kumar SharmaEdward W Inscho
May 16, 2001·American Journal of Physiology. Renal Physiology·S M WhiteE W Inscho
Jul 31, 2004·Acta Physiologica Scandinavica·E W InschoR J Evans
Mar 18, 2005·Physiology·Peter KomlosiP Darwin Bell
Jul 15, 2005·American Journal of Physiology. Cell Physiology·Yun Jung LeeHo Jae Han
Mar 26, 2010·American Journal of Physiology. Renal Physiology·David A Osmond, Edward W Inscho
Aug 23, 2019·Physiological Reviews·Volker VallonBellamkonda K Kishore
Mar 19, 2009·Purinergic Signalling·Edward W Inscho
Sep 14, 2017·Journal of the American Heart Association·Oleg PalyginAlexander Staruschenko
May 6, 2004·American Journal of Physiology. Renal Physiology·Zhong Zhang, Thomas L Pallone
Jun 7, 2003·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Armin Just, William J Arendshorst
Jan 9, 2009·American Journal of Physiology. Renal Physiology·Edward W InschoLi-Ming Jin
Jan 30, 2004·American Journal of Physiology. Renal Physiology·Peter KomlosiPhillip Darwin Bell
Mar 11, 2016·American Journal of Physiology. Renal Physiology·Fernando Augusto Malavazzi CasareMaria Oliveira-Souza
Apr 3, 2015·Physiological Reviews·Mattias CarlströmWilliam J Arendshorst
Jan 31, 2014·American Journal of Physiology. Renal Physiology·David A OsmondEdward W Inscho
Apr 18, 2020·Physiological Research·Z GuanE W Inscho
May 6, 2004·Journal of Hypertension·Edward W InschoJohn D Imig
Dec 29, 2020·Biochemical Pharmacology·Marie-Louise T MonaghanRobert J Unwin

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