Pressure perturbation calorimetry of apolipoproteins in solution and in model lipoproteins.

Proteins
Sangeeta Benjwal, Olga Gursky

Abstract

High-density lipoproteins (HDLs) are complexes of lipids and proteins (termed apolipoproteins) that remove cell cholesterol and protect from atherosclerosis. Apolipoproteins contain amphipathic alpha-helices that have high content (> or = 1/3) and distinct distribution of charged and apolar residues, adopt molten globule-like conformations in solution, and bind to lipid surfaces. We report the first pressure perturbation calorimetry (PPC) study of apolipoproteins. In solution, the main HDL protein, apoA-I, shows relatively large volume contraction, DeltaV(unf) = -0.33%, and an apparent reduction in thermal expansivity upon unfolding, Deltaalpha(unf) < or = 0, which has not been observed in other proteins. We propose that these values are dominated by increased charged residue hydration upon alpha-helical unfolding, which may result from disruption of multiple salt bridges. At 5 degrees C, apoA-I shows large thermal expansion coefficient, alpha(5 degrees) = 15.10(-4) K(-1), that rapidly declines upon heating from 5 to 40 degrees C, alpha(40 degrees) - alpha(5 degrees) = -4.10(-4) K(-1); apolipoprotein C-I shows similar values of alpha(5 degrees) and alpha(40 degrees). These values are larger than in globular proteins. They indic...Continue Reading

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