Pressure-temperature phase behavior of mixtures of natural sphingomyelin and ceramide extracts

Langmuir : the ACS Journal of Surfaces and Colloids
Hanna M G BarrigaNicholas J Brooks

Abstract

Ceramides are a group of sphingolipids that act as highly important signaling molecules in a variety of cellular processes including differentiation and apoptosis. The predominant in vivo synthetic pathway for ceramide formation is via sphingomyelinase catalyzed hydrolysis of sphingomyelin. The biochemistry of this essential pathway has been studied in detail; however, there is currently a lack of information on the structural behavior of sphingomyelin- and ceramide-rich model membrane systems, which is essential for developing a bottom-up understanding of ceramide signaling and platform formation. We have studied the lyotropic phase behavior of sphingomyelin-ceramide mixtures in excess water as a function of temperature (30-70 °C) and pressure (1-200 MPa) by small- and wide-angle X-ray scattering. At low ceramide concentrations the mixtures form the ripple gel phase (P(β)') below the gel transition temperature for sphingomyelin, and this observation has been confirmed by atomic force microscopy. Formation of the ripple gel phase can also be induced at higher temperatures via the application of hydrostatic pressure. At high ceramide concentration an inverse hexagonal phase (HII) is formed coexisting with a cubic phase.

References

Jan 18, 2003·Biochimica Et Biophysica Acta·Benjamin J PettusYusuf A Hannun
Nov 15, 2005·Biophysical Journal·Jesús SotAlicia Alonso
Jan 3, 2006·Biophysical Journal·Andrey FilippovGöran Lindblom
Feb 25, 2010·FEBS Letters·Branka Stancevic, Richard Kolesnick
Jul 2, 2010·The Review of Scientific Instruments·Nicholas J BrooksJohn M Seddon
Oct 26, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Pradeep UppamoochikkalJohn F Nagle
Dec 22, 2010·Chemistry and Physics of Lipids·Nicholas J BrooksJohn M Seddon
Mar 7, 2012·The Journal of Physical Chemistry. B·Rainer Metcalf, Sagar A Pandit
Oct 23, 2012·Nature Materials·Lobat TayebiAtul N Parikh
Jan 30, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Max LönnforsJ Peter Slotte
Feb 28, 2013·Biophysical Journal·Ibai ArtetxeTerhi Maula
Jun 19, 2013·Nature Medicine·Erich GulbinsJohannes Kornhuber
Dec 7, 2013·Langmuir : the ACS Journal of Surfaces and Colloids·Daniel M Carter RamirezLinda J Johnston
Feb 12, 2014·Progress in Lipid Research·Bruno M CastroLiana C Silva

❮ Previous
Next ❯

Citations

Oct 14, 2016·Colloids and Surfaces. B, Biointerfaces·F G DupuyR G Oliveira
Jun 8, 2017·Biophysical Journal·Mahmoudreza Doroudgar, Michel Lafleur
Sep 4, 2021·Journal of Physics. Condensed Matter : an Institute of Physics Journal·Nicholas J TerrillWim Bras

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis