Pressurized carbon dioxide as a potential tool for decellularization of pulmonary arteries for transplant purposes.

Scientific Reports
Alicia Gil-RamírezIrene Rodríguez-Meizoso

Abstract

Vascular bio-scaffolds produced from decellularized tissue offer a promising material for treatment of several types of cardiovascular diseases. These materials have the potential to maintain the functional properties of the extracellular matrix (ECM), and allow for growth and remodeling in vivo. The most commonly used methods for decellularization are based on chemicals and enzymes combinations, which often damage the ECM and cause cytotoxic effects in vivo. Mild methods involving pressurized CO2-ethanol (EtOH)-based fluids, in a supercritical or near supercritical state, have been studied for decellularization of cardiovascular tissue, but results are controversial. Moreover, data are lacking on the amount and type of lipids remaining in the tissue. Here we show that pressurized CO2-EtOH-H2O fluids (average molar composition, ΧCO2 0.91) yielded close to complete removal of lipids from porcine pulmonary arteries, including a notably decrease of pro-inflammatory fatty acids. Pressurized CO2-limonene fluids (ΧCO2 0.88) and neat supercritical CO2 (scCO2) achieved the removal of 90% of triacylglycerides. Moreover, treatment of tissue with pressurized CO2-limonene followed by enzyme treatment, resulted in efficient DNA removal. The...Continue Reading

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Citations

Aug 6, 2020·International Journal of Molecular Sciences·Unai MendibilAnder Abarrategi
Sep 4, 2021·Tissue Engineering. Part B, Reviews·Gustavo Henrique Doná Rodrigues AlmeidaMaria Angélica Miglino

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Methods Mentioned

BETA
differential scanning calorimetry
Assay
transmission electron microscopy
biopsy
PCA

Software Mentioned

MassLynx
LipidMaps®
SIMCA

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