Presynaptic architecture of the larval zebrafish neuromuscular junction

The Journal of Comparative Neurology
Frederik HelmprobstChristian Stigloher

Abstract

This article shows the ultrastructural architecture of larval zebrafish (Danio rerio) neuromuscular junctions in three dimensions. We compare classical electron microscopy fixation techniques with high-pressure freezing followed by freeze substitution (HPF/FS) in combination with electron tomography. Furthermore, we compare the structure of neuromuscular junctions in 4- and 8-dpf zebrafish larvae with HPF/FS because this allows for close-to-native ultrastructural preservation. We discovered that synaptic vesicles of 4-dpf zebrafish larvae are larger than those of 8-dpf larvae. Furthermore, we describe two types of dense-core vesicles and quantify a filamentous network of small filaments interconnecting synaptic vesicles as well as tethers connecting synaptic vesicles to the presynaptic cell membrane. In the center of active zones, we found elaborate electron-dense projections physically connecting vesicles of the synaptic vesicle pool to the presynaptic membrane. Overall this study establishes the basis for systematic comparisons of synaptic architecture at high resolution in three dimensions of an intact vertebrate in a close-to-native state. Furthermore, we provide quantitative information that builds the basis for diverse sy...Continue Reading

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Citations

Jun 9, 2015·Frontiers in Cellular Neuroscience·Joseph J BrucknerKate M O'Connor-Giles
Dec 17, 2016·Journal of Neurogenetics·Hong ZhanKate O'Connor-Giles
Dec 22, 2016·The Journal of Cell Biology·Joseph J BrucknerKate M O'Connor-Giles
Jan 6, 2017·PLoS Computational Biology·Kristin Verena KaltdorfChristian Stigloher
May 6, 2019·International Journal of Molecular Sciences·Rituparna Chakrabarti, Carolin Wichmann
Sep 12, 2018·EMBO Reports·Rituparna ChakrabartiCarolin Wichmann
Nov 7, 2019·Scientific Reports·Marie-Christin SpindlerRicardo Benavente
Oct 22, 2019·Communications Biology·Marie-Christin SpindlerRicardo Benavente
Oct 7, 2020·Proceedings of the National Academy of Sciences of the United States of America·Hua WenPaul Brehm
Jan 12, 2020·Communications Biology·Marie-Christin SpindlerRicardo Benavente

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