Presynaptic mitochondria in functionally different motor neurons exhibit similar affinities for Ca2+ but exert little influence as Ca2+ buffers at nerve firing rates in situ.

The Journal of Neuroscience : the Official Journal of the Society for Neuroscience
Amit K ChouhanGregory T Macleod

Abstract

Mitochondria accumulate within nerve terminals and support synaptic function, most notably through ATP production. They can also sequester Ca(2+) during nerve stimulation, but it is unknown whether this limits presynaptic Ca(2+) levels at physiological nerve firing rates. Similarly, it is unclear whether mitochondrial Ca(2+) sequestration differs between functionally different nerve terminals. We addressed these questions using a combination of synthetic and genetically encoded Ca(2+) indicators to examine cytosolic and mitochondrial Ca(2+) levels in presynaptic terminals of tonic (MN13-Ib) and phasic (MNSNb/d-Is) motor neurons in Drosophila, which, as we determined, fire during fictive locomotion at approximately 42 Hz and approximately 8 Hz, respectively. Mitochondrial Ca(2+) sequestration starts in both terminals at approximately 250 nM, exhibits a similar Ca(2+)-uptake affinity (approximately 410 nM), and does not require Ca(2+) release from the endoplasmic reticulum. Nonetheless, mitochondrial Ca(2+) uptake in type Is terminals is more responsive to low-frequency nerve stimulation and this is due to higher cytosolic Ca(2+) levels. Since type Ib terminals have a higher mitochondrial density than Is terminals, it seemed poss...Continue Reading

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Citations

Jun 24, 2011·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Dinara ShakiryanovaEdwin S Levitan
Jan 27, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Amit K ChouhanGregory T Macleod
Oct 5, 2012·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Jasper AkerboomLoren L Looger
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Apr 26, 2021·Mitochondrion·Sayantan Datta, Manish Jaiswal
Jul 4, 2012·Cold Spring Harbor Protocols·Gregory T Macleod

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