PMID: 9422380Jan 9, 1998Paper

Presynaptic modulation of cholecystokinin release by protein kinase C in the rat hippocampus

Journal of Neurochemistry
A I BreukelW E Ghijsen

Abstract

The role of protein kinase C (PKC) in modulating the release of the octapeptide cholecystokinin (CCK-8) was investigated in rat hippocampal nerve terminals (synaptosomes). The PKC-activating phorbol ester 4beta-phorbol 12,13-dibutyrate (beta-PDBu) dose dependently (5-5,000 nM) increased CCK-8 release in a strictly Ca2+dependent way. This effect was observed only when synaptosomes were stimulated with the K+(A) channel blocker 4-aminopyridine (4-AP; 1 mM) but not with KCl (10-30 mM). The PDBu-induced exocytosis of CCK-8 was completely blocked by the two selective PKC inhibitors chelerythrine and calphostin-C and was not mimicked by alpha-PDBu, an inactive phorbol ester. In addition, an analogue of the endogenous PKC activator diacylglycerol, oleoylacetylglycerol, dose dependently increased CCK-8 exocytosis. Beta-PDBu (50-100 nM) also stimulated the 4-AP-evoked Ca2+-dependent release of the classic transmitter GABA, which co-localizes with CCK-8 in hippocampal interneurons. As a possible physiological trigger for PKC activation, the role of the metabotropic glutamate receptor was investigated. However, the broad receptor agonist (1S,3R)-1-aminocyclopentane-1,3-dicarboxylic acid did not stimulate, but instead inhibited, both the C...Continue Reading

Citations

Aug 11, 2000·Journal of Neurochemistry·J Cartmell, D D Schoepp
Nov 13, 2001·Clinical and Experimental Pharmacology & Physiology·L Iannazzo
Dec 14, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Zhongling ZhengR Alberto Travagli
Feb 28, 2002·The European Journal of Neuroscience·A G Miriam LeendersWim E J M Ghijsen
Jun 16, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Shuxia WanR Alberto Travagli
Oct 23, 2010·Glia·Keiichi YasudaMasami Takahashi
Jul 18, 2001·Peptides·W E GhijsenV M Wiegant
Aug 21, 2003·Progress in Neurobiology·Mark J Millan

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