Presynaptic modulation of glutamate release targets different calcium channels in rat cerebrocortical nerve terminals

The European Journal of Neuroscience
E Vázquez, J Sánchez-Prieto

Abstract

We have studied which type/s of Ca2+-channel/s support glutamate exocytosis and its modulation by presynaptic receptors in cerebrocortical nerve terminals. Depolarization of nerve terminals with 30 mM KCl induced a Ca2+-dependent release of 3.64 +/- 0.25 nmol/mg of protein. The addition of either 2 microM omega-conotoxin-GVIA or 200 nM omega-agatoxin-IVA reduced the KCl-evoked release by 47.7 +/- 3.5% and 70.4 +/- 8.9% respectively, and by 85.7 +/- 4.1% when both toxins were co-applied. The activation of adenosine A1 receptors with N6-cyclohexyladenosine or the activation of metabotropic glutamate receptors with L(+)-2-amino-4-phosphonobutyrate inhibited the KCl-evoked release by 41.0 +/- 5.9 and 54.3 +/- 10% respectively. The extent of these inhibitions was not altered by the prior addition of 2 microM omega-conotoxin-GVIA but they were significantly enhanced when omega-agatoxin-IVA was added together with the adenosine A1 receptor agonist or the metabotropic glutamate receptor agonist, suggesting that omega-conotoxin-GVIA-sensitive and not omega-agatoxin-IVA-sensitive Ca2+-channels are involved in the action of these inhibitory receptors. By contrast, the facilitation of glutamate release that follows the activation of the pr...Continue Reading

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Citations

Jun 1, 2000·Progress in Neurobiology·A M Thomson
Sep 6, 2002·Neuroscience Letters·Carmelo Millán, José Sánchez-Prieto
Jul 5, 2011·Journal of Agricultural and Food Chemistry·Tzu Yu LinSu Jane Wang
Apr 15, 2014·European Journal of Pharmacology·Che-Chuan WangSu-Jane Wang
Aug 26, 2014·Neurochemical Research·Marion S Mercier, David Lodge
Mar 15, 2015·International Journal of Molecular Sciences·Tzu-Yu LinSu-Jane Wang
Oct 17, 2012·International Journal of Developmental Neuroscience : the Official Journal of the International Society for Developmental Neuroscience·Maike Hartlage-RübsamenSteffen Roßner
Jul 5, 2012·Toxicology and Applied Pharmacology·Tzu-Yu LinSu-Jane Wang
Apr 8, 2010·European Journal of Pharmacology·Hirohiko HikichiHisashi Ohta
Dec 9, 2008·Neurochemistry International·Yi Chang, Su-Jane Wang
May 30, 2008·Diabetes Research and Clinical Practice·Eiki Satoh, Ayako Takahashi
Jun 6, 2006·Journal of Neurochemistry·Mitja SchenningFrederic A Meunier
Oct 20, 2007·Journal of Neurochemistry·Carolina LaderaJosé Sánchez-Prieto
Oct 7, 2004·Neuroscience·S E Bradford, J V Nadler

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