Presynaptic P2X1-3 and α3-containing nicotinic receptors assemble into functionally interacting ion channels in the rat hippocampus

Neuropharmacology
Ricardo J RodriguesRodrigo A Cunha

Abstract

Previous studies documented a cross-talk between purinergic P2X (P2XR) and nicotinic acetylcholine receptors (nAChR) in heterologous expression systems and peripheral preparations. We now investigated if this occurred in native brain preparations and probed its physiological function. We found that P2XR and nAChR were enriched in hippocampal terminals, where both P2X1-3R and α3, but not α4, nAChR subunits were located in the active zone and in dopamine-β-hydroxylase-positive hippocampal terminals. Notably, P2XR ligands displaced nAChR binding and nAChR ligands displaced P2XR binding to hippocampal synaptosomes. In addition, a negative P2XR/nAChR cross-talk was observed in the control of the evoked release of noradrenaline from rat hippocampal synaptosomes, characterized by a less-than-additive facilitatory effect upon co-activation of both receptors. This activity-dependent cross-inhibition was confirmed in Xenopus oocytes transfected with P2X1-3Rs and α3β2 (but not α4β2) nAChR. Besides, P2X2 co-immunoprecipitated α3β2 (but not α4β2) nAChR, both in HEK cells and rat hippocampal membranes indicating that this functional interaction is supported by a physical association between P2XR and nAChR. Moreover, eliminating extracellular...Continue Reading

References

May 27, 1992·European Journal of Pharmacology·R H HenningA Den Hertog
Sep 1, 1990·Cellular and Molecular Neurobiology·V A EterovićM G McNamee
Feb 1, 1987·Journal of Neurochemistry·P J Richardson, S J Brown
Apr 24, 1989·Brain Research·A WieraszkoT N Seyfried
Jul 1, 1995·Naunyn-Schmiedeberg's Archives of Pharmacology·C AllgaierI von Kügelgen
May 1, 1995·British Journal of Pharmacology·V J BalcarM R Bennett
Mar 29, 1996·The Journal of Biological Chemistry·G E CartierJ M McIntosh
Feb 1, 1997·Trends in Neurosciences·S Wonnacott
Jun 1, 1997·Naunyn-Schmiedeberg's Archives of Pharmacology·H KochK Starke
Oct 16, 1999·Proceedings of the National Academy of Sciences of the United States of America·C LénaJ P Changeux
Apr 13, 2000·Neuropharmacology·R A Cunha, J A Ribeiro
Feb 24, 2001·Life Sciences·R A Cunha, J A Ribeiro
Dec 26, 2001·Neuropharmacology·Miguel Díaz-HernándezM Teresa Miras-Portugal
Sep 25, 2002·Physiological Reviews·R Alan North
Nov 19, 2002·Journal of Neurobiology·Michael W Quick, Robin A J Lester
Jan 22, 2004·Nature Reviews. Neuroscience·Holly S Engelman, Amy B MacDermott
Apr 7, 2004·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·J J Galligan, R A North
May 29, 2004·Trends in Pharmacological Sciences·Federico Dajas-Bailador, Susan Wonnacott
Oct 1, 2004·The Journal of Biological Chemistry·Eric Boué-GrabotMaurice Garret
May 26, 2005·Brain Research. Brain Research Reviews·Hai Wang, Xiulan Sun
Jun 28, 2005·Trends in Neurosciences·Rashid GiniatullinJerrel L Yakel
Jul 8, 2005·The Journal of Neuroscience : the Official Journal of the Society for Neuroscience·Ricardo J RodriguesRodrigo A Cunha
Dec 29, 2006·Journal of Molecular Neuroscience : MN·Ricardo J RodriguesRodrigo A Cunha
Dec 29, 2006·Journal of Molecular Neuroscience : MN·M Díaz-HernándezM T Miras-Portugal
Dec 17, 2009·Autonomic Neuroscience : Basic & Clinical·Fernando Ochoa-CortésCarlos Barajas-López
Apr 30, 2010·Neurogastroenterology and Motility : the Official Journal of the European Gastrointestinal Motility Society·D A Decker, J J Galligan
May 15, 2010·Molecular Brain·Keith Tully, Vadim Y Bolshakov

❮ Previous
Next ❯

Citations

Jul 6, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·R Alan North
Jan 18, 2017·Genesis : the Journal of Genetics and Development·Amy K Sater, Sally A Moody
Feb 22, 2018·Cell Death & Disease·Ana P SimõesRicardo J Rodrigues
Jul 28, 2019·International Journal of Molecular Sciences·Anna Pittaluga
Jun 16, 2021·Biochemical Pharmacology·R NeffD Bertrand
Jul 12, 2021·Molecular Neurobiology·Júlia Leão Batista SimõesMargarete Dulce Bagatini
Aug 8, 2021·International Journal of Molecular Sciences·Julian LommenAmira A H Ali

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.