PMID: 2997445Jan 1, 1985

Presynaptic regulation by ACh of the NE mediated responses in the rat vas deferens

Journal of Receptor Research
H F Miranda, W W Wolstenholme


In the isolated electrically stimulated vas deferens preparation the effect of exogenous acetylcholine (ACh) was studied. It was possible to differentiate two separate sites for the action of ACh. A postsynaptic effect (M1) which is revealed as a sudden decrease in the basal tension of the muscular twitch, antagonized competitively by atropine (pA2 = 8.44 +/- 0.79), potentiated at a ratio of 10.24 by neostigmine, and not altered by hexamethonium, yohimbine, clonidine, theophylline or prazosin. Treatment with reserpine or 6-OH-DA induced a supersensitivity of this effect. The second action is a presynaptic effect (M2), which is manifested by a rapid increase in the muscular twitch, is dose-dependent to ACh, and is potentiated significantly by reserpine, neostigmine and clonidine. This latter effect was not modified by atropine, hexamethonium, yohimbine, prazosin or theophylline. The normal efficacy of ACh is 185 times greater in presynaptic over postsynaptic receptors. The increase in the twitch is not considered to be by the release of endogenous ACh or ATP. The evidence presented here indicates that the presynaptic effect of ACh (M2) is due to participation of alpha 2-adrenoceptors in enhancement of the release of norepinephri...Continue Reading


Apr 1, 1977·Physiological Reviews·J A Nathanson
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Jun 15, 1980·Biochemical Pharmacology·B B Fredholm, P Hedqvist
Nov 1, 1981·Biochemical Pharmacology·E Richelson, E El-Fakahany
Jan 1, 1982·General Pharmacology·H F MirandaE H Fernández
Jan 1, 1983·General Pharmacology·E BustosC Paeile
Jun 1, 1980·Biochemical Pharmacology·S ListP Seeman


Nov 10, 2007·Naunyn-Schmiedeberg's Archives of Pharmacology·Pravesh SolankiThomas C Cunnane
Jan 1, 1987·General Pharmacology·H F MirandaG M Moreu
Jan 1, 1987·General Pharmacology·H F MirandaP A Santiago
Jan 1, 1988·General Pharmacology·H F MirandaP A Santiago
Jan 1, 1990·General Pharmacology·H SaavedraC Paeile
Mar 1, 1995·General Pharmacology·H F MirandaG Pinardi
Dec 1, 1994·General Pharmacology·H F MirandaG Pinardi
Jan 1, 1991·Comparative Biochemistry and Physiology. C, Comparative Pharmacology and Toxicology·D Bustamante, H F Miranda
Oct 29, 2002·Reproductive Toxicology·Yoshiki BanMatthew J van Zwieten
Jan 1, 1990·Journal of Receptor Research·H F MirandaD Bustamante
Jan 1, 1992·Journal of Receptor Research·H F MirandaG Pinardi
Feb 10, 2007·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Zibo ZhangAiping Wang
Apr 1, 1991·Neuropeptides·H F MirandaE H Fernández
Sep 20, 2005·British Journal of Pharmacology·Alina M CuprianThomas C Cunnane
Sep 12, 2015·Physiological Reports·Audrey WallaceGerard P Sergeant

Related Concepts

Acetylcholine Sulfate (1: 1)
Electric Stimulation Technique
Synaptic Transmission
Norepinephrine, (+, -)-Isomer
August Rats
Alpha-adrenergic receptor

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