Pretransplant CD4 helper function and interleukin 10 response predict risk of acute kidney graft rejection

Transplantation
R WeimerGerhard Opelz

Abstract

In a prospective study of 80 patients, we investigated the association of acute kidney graft rejection with pretransplant T helper/suppressor activity, B-cell responses, and in vitro cytokine secretion. Patients' CD4+ or CD8+ T cells were cocultured with control B cells and pokeweed mitogen for 6 days. SAC I was used for T cell- and monocyte-independent B-cell stimulation and pokeweed mitogen was used for T cell-dependent B-cell stimulation. B-cell differentiation was assessed in a reverse hemolytic plaque assay. Cytokine responses of T cells (interleukin [IL]-2, IL-10, gamma-interferon) and B cells/monocytes (IL-6, IL-8, tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor) were determined in culture supernatants using ELISA. Subsets of CD4+ T cells, CD8+ T cells, and B cells were assessed by flow cytometry. None of 12 patients with pretransplant CD4 helper defects (CD4 helper activity < 10%) had acute rejection episodes, in contrast to 32 of 68 (47%) patients with normal pretransplant CD4 helper function (P = 0.001). Patients with pretransplant CD4 helper defects also had better 1-year graft function than patients without CD4 helper defects (serum creatinine 1.2 +/- 0.1 mg/dl and 1.7 +/- 0.1 mg/dl, re...Continue Reading

References

Mar 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·F RoussetJ Banchereau
Jan 1, 1991·Annual Review of Immunology·M L Dustin, T A Springer
May 1, 1988·The Journal of Experimental Medicine·P PeveriM Baggiolini
Feb 28, 1989·Biochemical and Biophysical Research Communications·Y FurutaniK Matsushima
Jan 1, 1987·Annual Review of Cell Biology·A L DeFranco
Jan 1, 1987·Advances in Immunology·D F Jelinek, P E Lipsky
Oct 1, 1987·The Journal of Experimental Medicine·C P Maury, A M Teppo
Feb 1, 1984·The Journal of Experimental Medicine·L AndrusE Reich
Jan 1, 1995·Nephrology, Dialysis, Transplantation : Official Publication of the European Dialysis and Transplant Association - European Renal Association·R L Schmouder, S L Kunkel
Jan 1, 1994·Annual Review of Immunology·S Romagnani
Jan 1, 1994·Journal of Clinical Laboratory Analysis·J SteinmannW Müller-Ruchholtz
Mar 1, 1994·The Journal of Clinical Investigation·M S LeeN Sarvetnick

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Citations

Apr 24, 2001·Transplant Immunology·N H CartwrightE R Kaminski
Mar 12, 2004·Transplantation Proceedings·C SüsalG Opelz
Jan 15, 2009·International Urology and Nephrology·Mohsen NafarBehzad Eniollahi
Nov 1, 2008·Expert Opinion on Medical Diagnostics·Olaf Boenisch, Anil Chandraker
Jul 20, 1999·Kidney International·D SankaranI V Hutchinson
Dec 19, 2006·Transplantation Proceedings·C SpiridonA Nikaein
Feb 5, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Rolf WeimerGerhard Opelz
Aug 17, 2005·Transplant International : Official Journal of the European Society for Organ Transplantation·Vijayan SureshEdward Kaminski
Dec 2, 2008·Clinical Transplantation·Camelia SpiridonMichael Mack
Jun 15, 2006·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·R WeimerG Opelz
Nov 9, 2007·Clinical Transplantation·Afzal NikaeinMichael Mack
Mar 16, 2011·Transplant International : Official Journal of the European Society for Organ Transplantation·Rolf WeimerGerhard Opelz
Dec 16, 2009·Clinical Transplantation·Markus GollingArianeb Mehrabi
Jul 24, 2010·Nephrology·Katherine A BarracloughSteven J McTaggart
Jan 17, 2002·Transplantation·Steffen PelzlCaner Süsal
Apr 26, 2019·Analytical Cellular Pathology (Amsterdam)·Kristin MaiFranz Maximilian Rasche
Jun 1, 2002·Journal of the American Society of Nephrology : JASN·Caner SüsalGerhard Opelz

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