Pretreatment of Cardiac Stem Cells With Exosomes Derived From Mesenchymal Stem Cells Enhances Myocardial Repair

Journal of the American Heart Association
Zhiwei ZhangTakayuki Asahara

Abstract

Exosomes derived from mesenchymal stem cells (MSCs) were proved to boost cell proliferation and angiogenic potency. We explored whether cardiac stem cells (CSCs) preconditioned with MSC exosomes could survive and function better in a myocardial infarction model. DiI-labeled exosomes were internalized with CSCs. They stimulated proliferation, migration, and angiotube formation of CSCs in a dose-dependent manner. In a rat myocardial infarction model, MSC exosome-preconditioned CSCs had significantly better survival, enhanced capillary density, reduced cardiac fibrosis, and restored long-term cardiac function. MicroRNA profiling analysis revealed that a set of microRNAs were significantly changed in CSCs after MSC exosome treatment. Pretreatment of CSCs with MSC exosomes provided a promising strategy to improve survival and angiogenic potency of CSCs.

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Datasets Mentioned

BETA
GSE73598

Methods Mentioned

BETA
flow cytometry
Precipitation
protein assay
fluorescence microscopy
fluorescence activated cell sorting
FACS
light microscopy

Software Mentioned

Miranda
Affymetrix Expression Console
TreeView
Cluster
Pro Plus
Java
TargetScan
Image J
Prism
Image

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