PMID: 16622283Apr 20, 2006Paper

Pretreatment of corn stover by low-liquid ammonia recycle percolation process

Applied Biochemistry and Biotechnology
Tae Hyun KimJun-Seok Kim

Abstract

A pretreatment method using aqueous ammonia was investigated with the intent of minimizing the liquid throughput. This process uses a flowthrough packed column reactor (or percolation reactor). In comparison to the ammonia recycle percolation (ARP) process developed previously in our laboratory, this process significantly reduces the liquid throughput to one reactor void volume in packed bed (2.0-4.7 mL of liquid/g of corn stover) and, thus, is termed low-liquid ARP (LLARP). In addition to attaining short residence time and reduced energy input, this process achieves 59-70% of lignin removal and 48-57% of xylan retention. With optimum operation of the LLARP to corn stover, enzymatic digestibilities of 95, 90, and 86% were achieved with 60, 15, and 7.5 filter paper units/g of glucan, respectively. In the simultaneous saccharification and fermentation test of the LLARP samples using Saccharomyces cerevisiae (NREL-D5A), an ethanol yield of 84% of the theoretical maximum was achieved with 6% (w/v) glucan loading. In the simultaneous saccharification and cofermentation (SSCF) test using recombinant Escherichia coli (KO11), both the glucan and xylan in the solid were effectively utilized, giving an overall ethanol yield of 109% of th...Continue Reading

Citations

Mar 11, 2008·Applied Biochemistry and Biotechnology·Jun-Seok KimSoon-Chul Park
Aug 30, 2008·Applied Biochemistry and Biotechnology·D Y CorredorD Wang
Sep 5, 2009·Applied Biochemistry and Biotechnology·Chinnadurai Karunanithy, Kasiviswanathan Muthukumarappan
Nov 29, 2012·Applied Biochemistry and Biotechnology·Yuzhen ZhangHongzhang Chen
Nov 3, 2012·Journal of Biomedicine & Biotechnology·Anahita Dehkhoda EckardWilliam Gibbons
Sep 4, 2012·Bioresource Technology·Anahita Dehkhoda EckardWilliam Gibbons
Oct 22, 2008·Bioresource Technology·Jin Seop BakKyoung Heon Kim
Jul 11, 2009·Biotechnology and Bioengineering·Jin Seop BakKyoung Heon Kim
Jan 22, 2010·Biotechnology and Bioengineering·Zhihong Fu, Mark T Holtzapple
Jun 21, 2011·Enzyme Research·Gary BrodeurSubramanian Ramakrishnan
Sep 20, 2012·Bioresource Technology·Feng GuRichard Phillips
Jun 1, 2011·Biotechnology Advances·Valery B AgborDavid B Levin
Sep 10, 2011·Bioresource Technology·Chang Geun YooTae Hyun Kim
Apr 25, 2013·Bioresource Technology·Chan-Duck JungKyung-Sik Hong
Sep 6, 2015·Bioresource Technology·Jun Seok KimTae Hyun Kim
Sep 15, 2015·ChemSusChem·Marcos Henrique Luciano SilveiraLuiz Pereira Ramos
May 5, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Nguyen Phuong Vi Truong, Tae Hyun Kim
Sep 7, 2020·Environmental Pollution·Surbhi SharmaTejraj M Aminabhavi
Dec 4, 2019·Bioresource Technology·Chao ZhaoShishir P S Chundawat
Jan 15, 2017·Microbiology Spectrum·Miia R MäkeläKristiina Hildén

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