PMID: 11318037Apr 25, 2001Paper

Pretreatment with ammonia water for enzymatic hydrolysis of corn husk, bagasse, and switchgrass

Applied Biochemistry and Biotechnology
M KurakakeToshiaki Komaki

Abstract

Bagasse, corn husk, and switchgrass were pretreated with ammonia water to enhance enzymatic hydrolysis. The sample (2 g) was mixed with 1-6 mL ammonia water (25-28% ammonia) and autoclaved at 120 degreesC for 20 min. After treatment, the product was vacuum-dried to remove ammonia gas. The dried solid could be used immediately in the enzymatic hydrolysis without washing. The enzymatic hydrolysis was effectively improved with more than 0.5 and 1 mL ammonia water/g for corn husk and bagasse, respectively. In bagasse, glucose, xylose, and xylobiose were the main products. The adsorption of CMCase and xylanase was related to the initial rate of enzymatic hydrolysis. In corn husks, arabinoxylan extracted by pretreatment was substantially unhydrolyzed because of the high ratio of arabinose to xylose (0.6). The carbohydrate yields from cellulose and hemicellulose were 72.9% and 82.4% in bagasse, and 86.2% and 91.9% in corn husk, respectively. The ammonia/water pretreatment also benefited from switchgrass (Miscanthus sinensis and Solidago altissima L.) hydrolysis.

Citations

Oct 2, 2009·Journal of Industrial Microbiology & Biotechnology·D A SalviV Bazan
Apr 17, 2008·Applied Biochemistry and Biotechnology·Asli IsciRobert P Anex
Aug 22, 2008·Applied Biochemistry and Biotechnology·Asli IsciRobert P Anex
Feb 27, 2010·Applied Biochemistry and Biotechnology·Deepti A SalviVictor Bazan
Apr 27, 2010·Applied Biochemistry and Biotechnology·Chinnadurai Karunanithy, Kasiviswanathan Muthukumarappan
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