PMID: 11337097May 5, 2001Paper

Pretreatment with glucose increases entry of urocortin into mouse brain

Peptides
A J Kastin, V Akerstrom

Abstract

Although urocortin is a potent inhibitor of food ingestion after peripheral administration, it was recently shown that under normal conditions this peptide crosses the blood-brain barrier (BBB) at a very slow rate. We examined whether hyperglycemia could stimulate the rate of entry (K(i)) of (125)I-urocortin into the mouse brain. In euglycemic mice, (125)I-urocortin injected iv entered the brain at a rate similar to that of the vascular marker (99m)Tc-albumin. However, injection of glucose (3 g/kg, ip) 0.5, 1, or 2 h before the (125)I-urocortin greatly increased the influx of urocortin. Without the glucose, the self-inhibition characteristic of a saturable transport system was not apparent. Self-inhibition could be demonstrated after the glucose injection, indicating activation of a transport system for urocortin that was saturable. Injection of insulin (10 U/kg, ip) 1 or 2 h before the (125)I-urocortin decreased the K(i). Thus, the entry of urocortin into brain can be activated by changes in the concentration of blood glucose, illustrating the responsiveness of the BBB to regulatory influences.

References

May 1, 1996·The Journal of Clinical Investigation·R B HarrisR C Bruch
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May 6, 1998·Diabetes·M F SaadV Kamdar
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Mar 31, 2001·Hormone and Metabolic Research = Hormon- Und Stoffwechselforschung = Hormones Et Métabolisme·A J KastinW Pan

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Citations

Jul 31, 2003·The European Journal of Neuroscience·P SinnayahD A Denton
Dec 15, 2007·Progress in Neurobiology·Weihong Pan, Abba J Kastin
Aug 6, 2011·Neurobiology of Aging·Mickael DecressacAfsaneh Gaillard
Aug 7, 2004·Brain Research. Brain Research Reviews·Weihong Pan, Abba J Kastin
Apr 28, 2009·Progress in Neurobiology·Ivona BrasnjevicUNKNOWN European NanoBioPharmaceutics Research Initiative
Oct 20, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·L WangY Taché

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