Pretreatment with hyperoxia reduces in vivo infarct size and cell death by apoptosis with an early and delayed phase of protection

European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery
Mohsen FoadoddiniAli Khoshbaten

Abstract

Exposure to normobaric hyperoxia protects the heart against ischemia-reperfusion injury ex vivo. In the present study, we investigated the effect of the early and late phase of hyperoxia on in vivo myocardial infarction and apoptosis. Rats were exposed to room air preoxygenation (O(2)≥ 95%) followed by regional ischemia (30 min) and 0, 90, 180, and 360 min of reperfusion. Hyperoxic exposure was performed for 120 min either immediately or 24h before coronary occlusion followed by 360-min reperfusion. Infarct size was evaluated by Evans blue/triphenyltetrazolium chloride staining. Apoptosis in the infarcted area was evaluated by terminal deoxy-nucleotidyl transferase-mediated deoxy uridine triphosphate (dUTP) nick end-labeling (TUNEL). Caspase 3 activity was measured by fluorometric enzyme assay, Bcl-2 and Bax protein expression assessed by western blotting and DNA laddering assessed with DNA gel electrophoresis. The infarct size did not increase with increasing duration of reperfusion. However, apoptosis as evaluated by Bcl-2/Bax ratio, caspase 3 activity, and TUNEL-positive cells increased with increasing time of reperfusion. Both early and delayed pretreatment with hyperoxia reduced infarct size (p = 0.0013, p = 0.046), amelio...Continue Reading

References

Jan 21, 1997·Circulation·A SarasteL M Voipio-Pulkki
Mar 23, 2000·Cardiovascular Research·M NakamuraJ Vinten-Johansen
Apr 12, 2001·American Journal of Physiology. Heart and Circulatory Physiology·Z ChenB H Chua
Jul 11, 2001·Apoptosis : an International Journal on Programmed Cell Death·Z Q ZhaoJ Vinten-Johansen
Aug 14, 2001·Journal of the American College of Cardiology·G ValenG K Hansson
Jun 6, 2002·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·P TähepõldG Valen
Mar 27, 2003·The Journal of Thoracic and Cardiovascular Surgery·Peeter TähepõldGuro Valen
Feb 14, 2004·Cardiovascular Research·Frank EeftingPieter A Doevendans
Dec 23, 2004·Basic Research in Cardiology·Fausto LabrutoGuro Valen
Dec 30, 2004·Journal of Molecular and Cellular Cardiology·Richard N Kitsis, Douglas L Mann
Feb 22, 2005·Basic Research in Cardiology·Guro Valen, Jarle Vaage
Mar 21, 2006·Current Opinion in Anaesthesiology·Barbara Kabon, Andrea Kurz
Mar 28, 2006·Experimental Biology and Medicine·Hong ChoiMyung-Suk Kim
Dec 25, 2007·Experimental Biology and Medicine·Andreas RedelFranz Kehl
Jul 16, 2008·The Journal of Surgical Research·Mansour Esmaili DehajAli Khoshbaten

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Citations

Apr 10, 2013·Histochemistry and Cell Biology·Susi ZaraAndrea Porzionato
Jun 15, 2011·Molecular and Cellular Biochemistry·Carley Glass, Dinender K Singla
Aug 23, 2011·American Journal of Physiology. Heart and Circulatory Physiology·Carley Glass, Dinender K Singla
Apr 20, 2016·Life Sciences·Christopher DallMark G Angelos
Aug 15, 2014·Cardiovascular Toxicology·Zeinab JanahmadiMasoomeh Emamghoreishi
Nov 5, 2016·Clinics and Research in Hepatology and Gastroenterology·Jianjun YuLianbao Kong
Apr 5, 2017·Journal of Cellular and Molecular Medicine·Sean M DavidsonDerek M Yellon
Nov 15, 2011·Journal of the Renin-angiotensin-aldosterone System : JRAAS·Fatemeh SafariAli Khoshbaten
Oct 1, 2011·Cell Biochemistry and Biophysics·Ram Sudheer AdluriGautam Maulik

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