Pretreatment with the gap junction uncoupler heptanol does not limit infarct size in rabbit heart

Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology
A GysemberghK Przyklenk

Abstract

Previous findings indicate that heptanol, an agent well-recognized to disrupt chemical signaling between myocytes by uncoupling of gap junctions, significantly limited infarct size when administered at the time of reperfusion. Our aim was to assess on the potential role of cell--cell communication via gap junctions during ischemia by investigating whether "loading" the soon-to-be ischemic territory with heptanol would limit myocardial necrosis. Five isolated buffer-perfused rabbit hearts were pretreated with heptanol (0.5 mM) for 10 min, while 12 served as controls. In the final 30 s of treatment, a large marginal branch of the left circumflex coronary artery was occluded for 30 min followed by 2 h of reperfusion, and infarct size was delineated by tetrazolium staining. Heptanol had no significant effect on the extent of infarct: area of necrosis (AN, expressed as a percentage of the myocardium at risk) was 75+/-3% and 72+/-8% in vehicle- and heptanol-treated groups (P=.76). Thus, our results suggest that cell-to-cell communication via gap junctions during coronary artery occlusion does not contribute importantly to the development of necrosis in rabbit heart.

References

Dec 31, 1997·Journal of Neurosurgery·A RawanduzyM Nedergaard
Jul 23, 1999·Annals of the New York Academy of Sciences·K PrzyklenkR A Kloner
Jan 1, 1996·Journal of Thrombosis and Thrombolysis·S D BellowsR A Kloner

❮ Previous
Next ❯

Citations

Jun 15, 2007·The Journal of Membrane Biology·Lene Nygaard AxelsenMorten Schak Nielsen
May 29, 2002·Cardiovascular Pathology : the Official Journal of the Society for Cardiovascular Pathology·Guohu LiKarin Przyklenk
Aug 27, 2005·Clinical and Experimental Pharmacology & Physiology·Bao-Ping ChenQiang Xia
Jun 26, 2004·The Journal of Physiology·Antonio Rodriguez-SinovasJordi Soler-Soler
Jan 27, 2011·British Journal of Pharmacology·Elke De VuystLuc Leybaert
Feb 24, 2006·Journal of Cardiovascular Pharmacology·Ketil HauganJørgen Søberg Petersen
Feb 2, 2010·American Journal of Physiology. Heart and Circulatory Physiology·Tetsuji MiuraToshiyuki Yano
May 22, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Rainer SchulzGerd Heusch
Sep 18, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Uwe SchwankeGerd Heusch
Sep 23, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Tetsuji MiuraKazuaki Shimamoto
Jul 19, 2003·American Journal of Physiology. Heart and Circulatory Physiology·Ferran PadillaJordi Soler-Soler
Dec 14, 2005·The Journal of Pharmacology and Experimental Therapeutics·James K HennanDavid L Crandall
May 1, 2021·International Journal of Molecular Sciences·Antonio Rodríguez-SinovasIgnacio Ferreira-González

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.