Pretreatment with urea-hydrochloric acid enhances the isolation of Helicobacter pylori from contaminated specimens

Journal of Clinical Microbiology
Q SongB D Gold

Abstract

Human saliva seeded with H. pylori was incubated in urea-HCl and then cultured on nonselective media. Pretreatment with 0.06 N HCl-0.08 M urea for 5 min at 37 degrees C resulted in reproducible isolation of H. pylori, even at low inocula (< or =10(2) CFU/ml of saliva), despite the presence of large numbers of contaminating organisms.

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Citations

Nov 25, 2017·Experimental & Molecular Medicine·J K C Yee
Oct 12, 2001·Current Problems in Pediatric and Adolescent Health Care·B D Gold

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