Prevalence and Characteristics of Bacillus cereus Group Isolated from Raw and Pasteurised Milk

Current Microbiology
Behrad RadmehrEnzo A Palombo

Abstract

The elimination of spore-forming bacteria is not guaranteed by current pasteurisation processes and is a challenging problem for the dairy industry. Given that Bacillus cereus sensu lato (B. cereus group) is an important foodborne pathogen and spoiler in the dairy industry, this study aimed at evaluating the prevalence and characteristics of B. cereus group in raw and pasteurised milk samples collected in Victoria, Australia. Isolated B. cereus group were tested for antimicrobial susceptibility, biofilm formation and virulence properties. Genetic diversity was assessed using ERIC-PCR. Proteomic profiling using MALDI-TOF MS and chemical profiling using Fourier-transform infrared (FTIR) spectroscopy were also applied for clustering of the isolates. Results showed 42.3% of milk samples contained B. cereus group, with a higher contamination level for pasteurised milk. Virulence studies identified genes nheA, nheB, hblA and nheC in most isolates and cyk gene in 46% of all isolates. Antimicrobial susceptibility testing showed a high prevalence of resistance towards ampicillin, ceftriaxone and penicillin. The biofilm-forming capacity of our isolates showed that most (53.7%) had the ability to form a biofilm. Genetic profiling using ER...Continue Reading

References

Jul 1, 1989·Diagnostic Microbiology and Infectious Disease·C J Fernandes, V P Ackerman
Feb 1, 1997·International Journal of Food Microbiology·H D Larsen, K Jørgensen
Mar 3, 1997·International Journal of Food Microbiology·M C Te GiffelF M Rombouts
Jul 15, 1998·Current Microbiology·E HelgasonA B Kolstø
Apr 3, 2004·Letters in Applied Microbiology·S StepanovićM Svabić-Vlahović
Jan 5, 2007·Journal of Clinical Microbiology·Lucy J HathawayKathrin Mühlemann
May 21, 2009·FEMS Microbiology Reviews·Wenjun LiPierre-Edouard Fournier
Mar 17, 2010·Foodborne Pathogens and Disease·Sofroni EglezosNarelle Fegan
Mar 8, 2011·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A C M VelooE J Kuijper
Mar 29, 2011·International Journal of Food Microbiology·Jeane Quintanilha ChavesAdriana Marcos Vivoni
Jan 21, 2012·Journal of Microbiological Methods·Snehal JadhavEnzo A Palombo
Jun 27, 2013·International Journal of Food Microbiology·Sachin R KadamTjakko Abee
Aug 13, 2014·International Journal of Food Microbiology·Nuwan R VithanageNivedita Datta
Oct 6, 2014·Journal of Dairy Science·Catherine M McAuleyEdward M Fox
Mar 10, 2015·International Journal of Food Microbiology·Snehal JadhavEnzo A Palombo
Nov 22, 2017·International Journal of Food Microbiology·Davide PorcellatoHilde Mellegård
Jun 12, 2018·International Journal of Food Microbiology·Karin ArturssonEva Olsson Engvall
Nov 30, 2018·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Ângela NovaisLuísa Peixe

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
PCR
chips

Software Mentioned

MALDI Biotyper
IR Biotyper
SPECLUST
Bruker MALDI Biotyper
BioNumerics
Launchpad

Related Concepts

Related Feeds

Bacillus Cereus Infection

Bacillus Cereus is a gram-positive bacteria that is the cause of some foodborne illnesses and leads to diarrhea and vomiting. Discover the latest research on Bacillus Cereus Infection here.

Biofilms

Biofilms are adherent bacterial communities embedded in a polymer matrix and can cause persistent human infections that are highly resistant to antibiotics. Discover the latest research on Biofilms here.

Biofilm & Infectious Disease

Biofilm formation is a key virulence factor for a wide range of microorganisms that cause chronic infections.Here is the latest research on biofilm and infectious diseases.