Prevalence and concentration of non-tuberculous mycobacteria in cooling towers by means of quantitative PCR: a prospective study.

Current Microbiology
Bárbara AdradosJordi Morató

Abstract

There is an increasing level of interest in non-tuberculous mycobacteria (NTM) due to the increasing reported rates of diseases caused by them. Although it is well known that NTM are widely distributed in the environment it is necessary to identify its reservoirs to prevent possible infections. In this study, we aimed to investigate the occurrence and levels of NTM in cooling towers to provide evidences for considering these settings as possible sources of respiratory infections. In the current study, we detected and quantified the presence of NTM by means of a rapid method in water samples taken from 53 cooling towers of an urban area (Barcelona, Spain). A genus-specific quantitative PCR (Q-PCR) assay with a quantification limit (QL) of 500 cells l(-1) was used. 56% (30) of samples were positive with a concentration range from 4.6 × 10(3) to 1.79 × 10(6) cells l(-1). In some cases (9/30), samples were positive but with levels below the QL. The colonization rate confirmed that cooling towers could be considered as a potential reservoir for NTM. This study also evaluated Q-PCR as a useful method to detect and quantify NTM in samples coming from environmental sources.

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Citations

Apr 17, 2013·Canadian Journal of Microbiology·Noora H J RäsänenEila Torvinen
Jun 3, 2014·BMC Infectious Diseases·Michael P ChouRachel M Thomson
Aug 14, 2013·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Eila TorvinenJaana Kusnetsov
Dec 18, 2015·Microbiome·Andrew J HoisingtonChristopher A Lowry
Jun 5, 2015·Journal of Water and Health·Gaurav SaxenaAbhay Raj
Nov 11, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ashok AspatwarSeppo Parkkila

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