Prevalence and Detection of Trichomonas vaginalis in HIV-Infected Pregnant Women

Sexually Transmitted Diseases
Collin M PriceJeffrey D Klausner

Abstract

Trichomonas vaginalis is a sexually transmitted infection associated with increased transmission of HIV and significant adverse birth outcomes; culture and polymerase chain reaction (PCR) are commonly used in diagnosis. Consenting HIV-infected pregnant women were recruited from clinics in South Africa and screened for T. vaginalis using PCR. Polymerase chain reaction-positive women provided an additional sample for culture. We compared T. vaginalis detection between PCR and culture, and investigated how PCR cycle threshold (Ct) values differ among culture results. A total of 359 women were enrolled and 76 (20%) tested T. vaginalis PCR positive. Cultures were obtained from 61 of the PCR-positive women, and 38 (62%) were culture positive. The median baseline Ct of the PCR-positive/culture-positive group was 22.6 versus 38.0 among those who were PCR positive/culture negative (P < 0.001). Culture-positive cases had lower Ct values (higher DNA load); a Ct value less than 30 predicted positivity with a sensitivity of 97% and a specificity of 96%. Culture was positive in roughly half of PCR-positive cases. The culture-negative cases had significantly higher Ct values, indicating a lower concentration of T. vaginalis DNA. A Ct value of...Continue Reading

References

Aug 30, 2005·Infectious Diseases in Obstetrics and Gynecology·A M CaliendoG M Wingood
Dec 31, 2008·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Thomas A PetermanUNKNOWN RESPECT-2 Study Group
Nov 12, 2009·The Journal of Maternal-fetal & Neonatal Medicine : the Official Journal of the European Association of Perinatal Medicine, the Federation of Asia and Oceania Perinatal Societies, the International Society of Perinatal Obstetricians·Joshua R MannTariq Gill
Mar 26, 2010·Journal of Perinatology : Official Journal of the California Perinatal Association·F Z GumboB Stray-Pedersen
Apr 23, 2013·Sexually Transmitted Infections·Patricia Kissinger, Alys Adamski
May 16, 2014·Sexually Transmitted Diseases·Bronwyn J SilverAlice R Rumbold
Oct 30, 2016·International Journal of STD & AIDS·Oyetunde T OyeyemiIfeoluwa T Oyeyemi
Oct 23, 2015·Journal of Clinical Microbiology·Barbara Van Der Pol
Nov 26, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Kimberly A Workowski

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Citations

Dec 4, 2019·Current Opinion in Infectious Diseases·Christina A MuznyPatricia Kissinger
Oct 5, 2019·F1000Research·Olivia T Van Gerwen, Christina A Muzny
Feb 23, 2020·International Journal of STD & AIDS·Nonkululeko MabasoNathlee Abbai
Dec 18, 2021·Southern African Journal of Infectious Diseases·Bongekile Ngobese, Nathlee S Abbai

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