Prevalence and distribution of Entamoeba species in a rural community in northern South Africa

Food and Waterborne Parasitology
Amidou SamieAli ElBakri

Abstract

Amoebiasis occurs worldwide and affects about 20-50 million people annually. Stool samples were collected from patients attending different rural clinics in Northern South Africa in the present study. Microscopic examination was performed for the initial detection of Entamoeba parasites. A multiplex PCR protocol based on the small subunit rRNA gene of E. moshkovskii, E. dispar, and E. histolytica, was used for the differential detection of the three Entamoeba species (collectively referred to as Entamoeba complex). A total of 170 participants were recruited in the study, with a mean age of 35.9 ± 17.8 years and a median of 37.0 years. The prevalence of Entamoeba species was found to be 34.7% and 33% by PCR and microscopy, respectively. E. histolytica had a prevalence of 4.1%, E. dispar 14.7% and E. moshkovskii 15.9%. Of the three species, only E. histolytica was significantly associated with diarrhoea and was more prevalent among HIV patients even in the absence of diarrhoea while the other two were not, although the difference was not significant (p > 0.05). This is the first study in South Africa to describe the prevalence of E. moshkovskii. E. dispar was significantly associated with abdominal pains (p = 0.003). Further stud...Continue Reading

References

May 10, 2003·Emerging Infectious Diseases·Ibne Karim M AliC Graham Clark
Jul 2, 2004·Transactions of the Royal Society of Tropical Medicine and Hygiene·Amha KebedeTon Polderman
Jun 16, 2005·Experimental Parasitology·Patricia MoranCecilia Ximénez
Sep 7, 2006·Journal of Clinical Microbiology·Zulhainan HamzahPorntip Chavalitshewinkoon-Petmitr
Oct 25, 2007·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·R FotedarJ Harkness
May 13, 2008·Acta Tropica·David L BeckEric R Houpt
Jan 15, 2011·The Korean Journal of Parasitology·Hassan Ahmed Hassan Ahmed IsmailYoung-Ha Lee
Mar 15, 2011·Epidemiology and Infection·J YakoobW Jafri
Jul 7, 2011·Journal of Global Infectious Diseases·Cecilia XiménezPartida Oswaldo
Jun 23, 2012·The Journal of Infectious Diseases·Chikako ShimokawaShinjiro Hamano
Oct 2, 2015·BMC Public Health·Zandile J MchizaDemetre Labadarios

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
electrophoresis
ELISA

Software Mentioned

SPSS

Related Concepts

Related Feeds

Amoebiasis

Amoebiasis, infection by the protozoan parasite Entamoeba histolytica, remains a global health problem, despite the availability of effective treatment. Here is the latest research.