Prevalence and diversity of integrons and associated resistance genes in faecal Escherichia coli isolates of healthy humans in Spain

The Journal of Antimicrobial Chemotherapy
Laura VinuéCarmen Torres

Abstract

To analyse the prevalence and diversity of integrons in faecal Escherichia coli isolates from healthy humans in Spain. One hundred E. coli isolates were obtained in Levine agar plates from faecal samples of 100 healthy humans during March to October 2007. Susceptibility to 16 antimicrobial agents was determined by the disc diffusion method. The presence and characterization of class 1, 2 and 3 integrons, as well as the presence of other antimicrobial resistance genes, were performed by PCR and DNA sequencing. Integrases associated with class 1 and/or class 2 integrons were identified in 29 E. coli isolates (intI1 gene in 26 isolates, intI2 in 1 isolate and intI1 + intI2 in 2 isolates), the remaining 71 isolates being free of these integrons. Seven different gene cassette arrangements were demonstrated in 27 of the 28 intI1-positive isolates and were as follows (number of isolates): dfrA1 + aadA1 (12), aadA (8), dfrA17 + aadA5 (3), dfrA7 (1), dfrA5 (1), dfrA1 (1) and dfrA12 + orfF + aadA2 (1). Four isolates presented defective class 1 integrons lacking the 3'-conserved region. The three isolates containing class 2 integrons harboured the dfrA1 + sat + aadA1 gene cassette array in their variable region. Integron-positive isolates...Continue Reading

References

Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Sep 25, 2004·Antimicrobial Agents and Chemotherapy·Jae DuganArthur A Andersen
Mar 24, 2005·International Journal of Antimicrobial Agents·Berónica InfanteGöran Kronvall
Apr 26, 2007·FEMS Immunology and Medical Microbiology·Stefano CocchiJean-Claude Piffaretti

❮ Previous
Next ❯

Citations

Aug 14, 2010·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Maria Cecilia Cergole-NovellaAntonio C C Pignatari
May 27, 2011·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Yanping LuoYue Ma
Aug 23, 2011·Applied and Environmental Microbiology·Maria KarczmarczykSéamus Fanning
Apr 17, 2013·BMC Microbiology·Belén Ruiz del CastilloLuis Martínez-Martínez
Jan 24, 2014·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Yuan XueYuping Hua
Jul 31, 2010·Journal of Medical Microbiology·Jannine K BaileyRuth M Hall
Dec 7, 2010·International Journal of Food Microbiology·Leila SoufiCarmen Torres
Apr 5, 2015·Poultry Science·Lara CavicchioAlessandra Piccirillo
Nov 8, 2014·Osong Public Health and Research Perspectives·Mohammad KargarAlireza Japoni-Nejad
Dec 4, 2010·Research in Microbiology·Maria Cecilia Cergole-NovellaBeatriz Ernestina Cabilio Guth
Mar 19, 2016·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Natália CanalGertrudes Corção
Aug 18, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Joel ManyahiBjørn Blomberg
Feb 18, 2014·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M SoléJ Vila
Jan 8, 2013·Veterinary Microbiology·Mauricio MarchantMiguel A Moreno
Mar 13, 2016·Journal of the Science of Food and Agriculture·Enrique Víctor Navajas-BenitoCarmen Torres
Feb 14, 2017·The Journal of Veterinary Medical Science·Yeon Soo ChungKun Taek Park
May 28, 2014·Revista Da Sociedade Brasileira De Medicina Tropical·Alexsandra Maria Silva LimaAna Catarina Souza Lopes
Jan 10, 2018·Frontiers in Microbiology·Nancy de Castro StoppeTatiana T Torres
Jan 17, 2020·Pakistan Journal of Biological Sciences : PJBS·Mukhtar A Al-HammadiIbrahim Al-Suboal
Oct 22, 2015·Annals of Clinical Microbiology and Antimicrobials·Yang DengGuangchao Yu
Oct 24, 2018·International Journal of Environmental Research and Public Health·Lara Pérez-EtayoAna Isabel Vitas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antifungals

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Antifungals (ASM)

An antifungal, also known as an antimycotic medication, is a pharmaceutical fungicide or fungistatic used to treat and prevent mycosis such as athlete's foot, ringworm, candidiasis, cryptococcal meningitis, and others. Discover the latest research on antifungals here.