Prevalence and genetic characterization of Toxoplasma gondii in pet dogs in Central China

The Korean Journal of Parasitology
Weifeng QianChaochao Lv

Abstract

The prevalence and genotype of Toxoplasma gondii infection in dogs in Henan Province, Central China was investigated. A total of 125 blood samples were collected from pet dogs during April to June 2013, and all samples were examined by indirect hemagglutination antibody test (IHA) and nested PCR. The overall T. gondii prevalence in pet dogs was 24.0% (30/125), with 20.8% (26/125) in IHA and 10.4% (13/125) in PCR, respectively. No statistical associations were found between animal gender and age and the prevalence of T. gondii infection. Thirteen positive DNA samples were genotyped using 11 PCR-RFLP markers, including SAG1, (3'+5') SAG2, alt.SAG2, SAG3, BTUB, GRA6, c22-8, c29-2, L358, PK1, and Apico. Of these, only 2 samples were genotyped with complete data for all loci, and a novel genotype (type III at SAG3 and GRA6 loci, and type I at other loci) was identified. This is the first report of genetic characterization of T. gondii infection in dogs in China.

Citations

Sep 2, 2015·The Korean Journal of Parasitology·Hai-Hai JiangXing-Quan Zhu
Jun 15, 2019·Parasite : Journal De La Société Française De Parasitologie·Weifeng QianTianqi Wang
Oct 21, 2016·Parasite : Journal De La Société Française De Parasitologie·Fen LiGuo-Hua Liu
Jul 9, 2020·International Journal of Environmental Research and Public Health·Mohammed Nasiru WanaRoslaini Abd Majid
Nov 8, 2020·Veterinary Parasitology·Jitender P DubeyChunlei Su

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Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

SPSS

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