Prevalence and genotypes of Giardia duodenalis in 1-2 year old dairy cattle

Veterinary Parasitology
James M TroutRonald Fayer

Abstract

To determine the prevalence of Giardia genotypes in 12-24 month old dairy heifers, fecal specimens were collected from two farms each in Vermont, New York, Pennsylvania, Maryland, Virginia, North Carolina, and Florida. Specimens, cleaned of fecal debris and concentrated using CsCl density gradient centrifugation, were subjected to PCR and DNA sequence analysis. Prevalence of Giardia infection, ranged from 11% to 75% on 14 farms with an average prevalence of 36% (204 positive cattle out of 571 examined). DNA sequence analysis of the 16S rRNA gene revealed 91% of the 204 Giardia isolates were Assemblage E, and 9% were Assemblage A. The prevalence of these genotypes varied greatly from farm to farm, with four farms having exclusively Assemblage E Giardia. Overall, Assemblage E was present in 33% of all animals tested and Assemblage A was present in 3% of the animals. Thus, while many of the heifers were infected with a genotype that is not known to be infectious for humans, 1-2 year old heifers on 10 of 14 farms did harbor zoonotic Assemblage A Giardia. Therefore, heifers cannot be overlooked as a potential source of human infectious cysts in the environment, with some farms representing a much higher risk than others.

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Sep 24, 2004·Veterinary Parasitology·James M TroutRonald Fayer
Jun 1, 2005·Veterinary Parasitology·James M TroutRonald Fayer

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Citations

Mar 24, 2012·Folia Parasitologica·Flávio Medeiros Paz e SilvaJoão Pessoa Araújo
Jun 24, 2008·Applied and Environmental Microbiology·Cynthia L WinkworthColin R Townsend
Apr 28, 2009·Epidemiology and Infection·S J SnelJ Learmonth
Jul 17, 2013·International Journal for Parasitology·Una Ryan, Simone M Cacciò
Mar 14, 2008·Parasitology Research·Yaoyu FengLihua Xiao
Sep 14, 2012·Molecular and Cellular Probes·Yvonne A L LimRobin B Gasser
Mar 17, 2009·Experimental Parasitology·Thomas GeurdenEdwin Claerebout
Mar 7, 2009·Veterinary Parasitology·Mónica SantínRonald Fayer
Jan 13, 2009·Trends in Parasitology·Paul T MonisR C Andrew Thompson
Apr 25, 2007·Veterinary Parasitology·Carla MendonçaNuno Canada
Mar 12, 2016·Environmental Science and Pollution Research International·Kareem Hatam-NahavandiMostafa Rezaeian
Jun 15, 2007·Veterinary Parasitology·James M TroutRonald Fayer
Oct 14, 2011·Veterinary Parasitology·Miguella P Mark-CarewHussni O Mohammed
Jun 23, 2010·Acta Veterinaria Scandinavica·Miguella P Mark-CarewHussni O Mohammed
Mar 6, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Fabienne D UehlingerBrent R Dixon
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Nov 8, 2011·Journal of Food Protection·Ebo Budu-AmoakoJ T McClure
Jan 15, 2011·Clinical Microbiology Reviews·Yaoyu Feng, Lihua Xiao

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