Prevalence and genotypic identification of Cryptosporidium spp. and Enterocytozoon bieneusi in captive Asiatic black bears (Ursus thibetanus) in Heilongjiang and Fujian provinces of China

BMC Veterinary Research
Sheng-Nan WangWei Zhao

Abstract

Cryptosporidium and Enterocytozoon bieneusi are two important pathogens with zoonotic potential that cause enteric infections in a wide range of hosts, including humans. Both are transmitted from animals to humans by direct contact or through contaminated equipment. Bears are frequently found in Chinese zoos as ornamental animals as well as farmed as commercial animals, and are therefore in close contact with zoo- or farm-keepers, but the prevalence and zoonotic potential of Cryptosporidium and E. bieneusi in bears is poorly understood. In this study, we aimed to provide data on the occurrence and genetic diversity of Cryptosporidium and E. bieneusi in Asiatic black bears from Heilongjiang and Fujian, China. From May 2015 to December 2017, 218 fresh fecal specimens were collected from captive Asiatic black bears in Heilongjiang (n = 36) and Fujian (n = 182), China. Cryptosporidium and E. bieneusi were examined by PCR amplification of the partial small subunit of ribosomal DNA (SSU rDNA) and the internal transcribed spacer (ITS) region of rDNA, respectively. C. andersoni-positive isolates were subtyped through PCR analysis of the four minisatellite/microsatellite (MS1, MS2, MS3 and MS16) loci. The overall prevalence of Cryptospo...Continue Reading

References

Apr 2, 1999·Applied and Environmental Microbiology·L XiaoA A Lal
May 8, 1999·Journal of Wildlife Diseases·R B DuncanH D Moll
Dec 29, 2000·The Journal of Parasitology·L XiaoA A Lal
Sep 28, 2001·Journal of Clinical Microbiology·K GuyotE Dei-Cas
Apr 27, 2002·Applied and Environmental Microbiology·Michael A BuckholtSaul Tzipori
Aug 9, 2005·Applied and Environmental Microbiology·Jianlin JiangLihua Xiao
Jan 17, 2007·Epidemiology and Infection·T D MorseH V Smith
Apr 2, 2009·The Journal of Eukaryotic Microbiology·Mónica Santín, Ronald Fayer
Jul 12, 2011·Parasitology Research·Petra RavaszovaMilos Halan
Sep 13, 2011·Applied and Environmental Microbiology·Liette S WaldronMichelle L Power
Aug 10, 2012·AIDS Research and Human Retroviruses·Mahmoud AgholiMohammad Hossein Motazedian
Oct 24, 2012·Journal of Parasitology Research·Olga MatosLihua Xiao
Oct 22, 2013·Applied and Environmental Microbiology·Yaqiong GuoLihua Xiao
Oct 4, 2014·The Lancet Infectious Diseases·William CheckleyEric R Houpt
Jul 21, 2015·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Guang-Hui ZhaoXing-Quan Zhu
Oct 4, 2016·Veterinary Parasitology·Nawal HijjawiUna Ryan
Dec 20, 2016·Foodborne Pathogens and Disease·Cheyenne C ConradTim McAllister
Jan 20, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·G HussainJ Paul
Jun 30, 2018·Parasites & Vectors·Yan ZhangRobin B Gasser
Aug 16, 2018·Trends in Parasitology·Yaoyu FengLihua Xiao
Apr 14, 2019·European Journal of Protistology·Nikola HolubováMartin Kváč
Apr 29, 2019·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Haiyan WangLongxian Zhang

❮ Previous
Next ❯

Citations

Sep 24, 2020·International Journal of Environmental Research and Public Health·Shahira A Ahmed, Panagiotis Karanis

❮ Previous
Next ❯

Methods Mentioned

BETA
genotyping
PCR
PCRs
electrophoresis

Software Mentioned

Basic Local Alignment Search Tool ( BLAST )
Social Sciences ( SPSS )
ClustalX

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.