Prevalence and molecular characterization of some circulating strains of the peste-des-petits -ruminants virus in Saudi Arabia between 2014-2016

PeerJ
Maged Gomaa HemidaSayed Ali

Abstract

The peste-des-petits-ruminants virus (PPRV) is a highly devastating virus of small ruminants in many parts of the world, including the Kingdome of Saudi Arabia. Therefore, our objectives were (1) to conduct a molecular prevalence study of PPRV in sheep and goat across the KSA and (2) to isolate and identify currently circulating PPRV lineages. Swabs and tissue specimens were collected from 97 herds suspected to be infected with PPRV across the Kingdome of Saudi Arabia (KSA). Testing for the presence of the virus was done by the real-time PCR. Confirmation of the identity of the reactions was done by the gel-based-PCR then by sequencing of the partial PPRV genome. Our results revealed that 24.1% of the tested specimens were PPRV-positive. Isolation of PPRV was successful from samples using the Vero cell line. Sequence analysis of some partial PPRV genes (N, F, M, L, P, and H) revealed that these strains were belonging to lineage IV of the PPRV. This is the first study to conduct both the nationwide prevalence, isolation, and molecular characterizations of the PPRV in the KSA. Continuous surveillance and monitoring of the circulating strains of PPRV among sheep and goats will contribute substantially to the global eradication cam...Continue Reading

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Citations

Nov 26, 2021·Veterinary Sciences·Lingxia LiYoujun Shang

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Datasets Mentioned

BETA
KY798143
KJ867541.1
KY679139
MT421967
FR667645.1
FN995267.1
KJ867545.1
MN369542

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Mega
blastn
Trimmomatic
Trinity
FastQC
Statistical Packages for Social Sciences [ SPSS ]

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