Prevalence and pathogenic potential of Escherichia coli isolates from raw milk and raw milk cheese in Egypt

International Journal of Food Microbiology
Rabee A OmbarakShinji Yamasaki

Abstract

The objectives of this study were to investigate prevalence and pathogenic potential of Escherichia coli contaminating raw milk and its products in Egypt. Out of 187 dairy products including 72 raw milk samples, 55 Karish cheese and 60 Ras cheese, 222 E. coli isolates including 111, 89 and 22 were obtained from 55 raw milk samples (76.4%), 41 Karish cheese (74.5%), and 13 Ras cheese (21.7%), respectively. Isolated E. coli strains were examined for 24 representative virulence genes present in diarrheagenic E. coli (DEC) and extraintestinal pathogenic E. coli (ExPEC). Among DEC and ExPEC virulence factors, genes for enteropathogenic E. coli (eaeA, bfpA, EAF), enterohemorrhagic E. coli (stx1, stx2, eaeA), enterotoxigenic E. coli (elt, est), enteroinvasive E. coli (invE), enteroaggregative E. coli (Eagg, astA), diffusely adherent E. coli (daaD), ExPEC (cdt-I to cdt-V, cnf1, cnf2, hlyA) and putative adhesins (efa1, iha, ehaA, saa, and lpfAO113) were screened by colony hybridization assay. Out of 222 E. coli strains, 104 (46.8%) isolated from 69 (36.9%) samples carried one or more virulence genes. The most prevalent gene detected was lpfAO113 (40.5%), followed by ehaA (32.4%,), astA (3.15%,), iha (1.80%), hlyA (1.35%), stx1 (0.90%), ...Continue Reading

References

Sep 1, 1987·The Pediatric Infectious Disease Journal·J P NataroM M Levine
Oct 1, 1995·FEMS Immunology and Medical Microbiology·S YamamotoO Yoshida
Apr 1, 1997·Letters in Applied Microbiology·E J Quinto, A Cepeda
Apr 30, 1999·Emerging Infectious Diseases·Y NishikawaK Haruki
Sep 30, 2000·Applied and Environmental Microbiology·O ClermontE Bingen
Oct 24, 2000·International Journal of Medical Microbiology : IJMM·P Klemm, M A Schembri
Aug 3, 2001·International Journal of Food Microbiology·M L De BuyserV Lafarge
Mar 26, 2004·Nature Reviews. Microbiology·James B KaperHarry L Mobley
Nov 6, 2004·Journal of Clinical Microbiology·Claudia TomaMarta Rivas
Mar 7, 2008·Journal of Clinical Microbiology·Chase E GuionThomas G Cleary
Feb 16, 2010·International Journal of Food Microbiology·Laura Cristina RúgelesOscar Gilberto Gómez-Duarte
Jan 5, 2011·Infection and Immunity·Cynthia A FullerAlison A Weiss
Mar 3, 2011·FEMS Immunology and Medical Microbiology·Louise BélangerCharles M Dozois
Jul 5, 2012·Journal of Clinical Microbiology·Flemming ScheutzAlison D O'Brien
Oct 5, 2013·Clinical Microbiology Reviews·Matthew A CroxenB Brett Finlay
May 1, 2015·Journal of Clinical Microbiology·Atsushi IguchiUNKNOWN Pathogenic E. coli Working Group in Japan

❮ Previous
Next ❯

Citations

Jun 1, 2016·Frontiers in Microbiology·Atsushi IguchiTetsuya Hayashi
Feb 17, 2017·Letters in Applied Microbiology·C A AlonsoC Torres
Dec 1, 2017·Veterinary World·Farhad Sharafati ChaleshtoriReza Sharafati Chaleshtori
Dec 8, 2017·Foodborne Pathogens and Disease·Anna C L P de CamposGerson Nakazato
Feb 14, 2020·International Journal of Environmental Health Research·Ahmed S AhmedAhmed Ezzat Ahmed
Jul 10, 2019·Food Science and Technology International = Ciencia Y Tecnología De Los Alimentos Internacional·Pavel AčaiL'ubomír Valík
Apr 9, 2019·Journal of Food Science and Technology·Darla Silveira Volcan MaiaWladimir Padilha da Silva
Nov 26, 2019·Journal of Environmental Science and Health. Part. B, Pesticides, Food Contaminants, and Agricultural Wastes·Mahmoud ElafifyAdel Abdelkhalek
Dec 18, 2020·Journal of Clinical Microbiology·Thi Thu Huong NguyenSunao Iyoda
May 6, 2021·Comprehensive Reviews in Food Science and Food Safety·Jonathan B MaganNoel A McCarthy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.