Prevalence and patterns of rifampicin and isoniazid resistance conferring mutations in Mycobacterium tuberculosis isolates from Uganda

PloS One
Edgar KigoziDavid Patrick Kateete

Abstract

Accurate diagnosis of tuberculosis, especially by using rapid molecular assays, can reduce transmission of drug resistant tuberculosis in communities. However, the frequency of resistance conferring mutations varies with geographic location of Mycobacterium tuberculosis, and this affects the efficiency of rapid molecular assays in detecting resistance. This has created need for characterizing drug resistant isolates from different settings to investigate frequencies of resistance conferring mutations. Here, we describe the prevalence and patterns of rifampicin- and isoniazid- resistance conferring mutations in isolates from Uganda, which could be useful in the management of MDR-TB patients in Uganda and other countries in sub-Saharan Africa. Ninety seven M. tuberculosis isolates were characterized, of which 38 were MDR, seven rifampicin-resistant, 12 isoniazid-mono-resistant, and 40 susceptible to rifampicin and isoniazid. Sequence analysis of the rpoB rifampicin-resistance determining region (rpoB/RRDR) revealed mutations in six codons: 588, 531, 526, 516, 513, and 511, of which Ser531Leu was the most frequent (40%, 18/45). Overall, the three mutations (Ser531Leu, His526Tyr, Asp516Tyr) frequently associated with rifampicin-res...Continue Reading

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Citations

May 6, 2020·Lung India : Official Organ of Indian Chest Society·Ashok Singh CharanManisha Singh
Nov 13, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Saba KabirAbdul Rehman
Apr 21, 2021·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Zoi FlorouEfi Petinaki
May 1, 2021·Pharmaceutics·Filia StephanieUsman Sumo Friend Tambunan
Jun 3, 2021·Tropical Medicine and Infectious Disease·Mark Tefero Kivumbi, Claire J Standley

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Methods Mentioned

BETA
PCRs
electrophoresis
PCR

Software Mentioned

GeneXpert
Translate
Molecular Evolutionary Genetics Analysis
BLAST
translated
MEGA6
BioEdit
ExPASy

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