Prevalence and sequence analysis of equid herpesviruses from the respiratory tract of Polish horses

Virology Journal
Karol StasiakJerzy Rola

Abstract

Equid herpesviruses (EHVs) are widespread in equine populations worldwide. While the infection with equine α-herpesviruses (EHV-1 and EHV-4) has been linked to several clinical outcomes, the pathogenic potential for equine γ-herpesviruses (EHV-2 and EHV-5) is still unclear. The objective of the current study was to determine the prevalence of infection with EHVs among Polish horses, to investigate factors associated with EHV infections among horses sampled, and to determine genetic variability within Polish EHV-2 isolates. Virus-specific real-time PCR assays were used for detection of EHV-1, EHV-2, EHV-4 and EHV-5 in nasal swabs collected from 540 horses from 13 national horse studs located throughout Poland. A proportion of EHV-2/5 positive samples were subjected to virus isolation followed by amplification and analysis of partial glycoprotein B sequence. Overall, 448/540 (83.0%) horses sampled were positive for at least one virus. The most prevalent was infection with EHV-2 (77.2%), followed by EHV-5 (47.0%), and EHV-4 (0.4%). None of the horses was positive for EHV-1. Approximately half of the virus-infected horses were positive for both EHV-2 and EHV-5. The proportion of EHV-2/5 positive horses varied by age, breed, and sea...Continue Reading

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Citations

Jun 13, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Alais Maria Dall AgnolAmauri Alcindo Alfieri
Oct 8, 2020·BMC Veterinary Research·Karol StasiakJerzy Rola
Sep 15, 2021·Equine Veterinary Journal·Charlotte Easton-Jones

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Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

BioEdit
GraphPad Prism
MEGA5
GraphPad

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