Prevalence and spread of enterohepatic Helicobacter species in mice reared in a specific-pathogen-free animal facility

Journal of Clinical Microbiology
Ulrich R M BohrPeter Malfertheiner

Abstract

Infections with enterohepatic Helicobacter species (EHS) can change the results of animal experiments. However, there is little information about the prevalence of EHS in noncommercial animal facilities. The aim of this study was to investigate the prevalence and the spread of EHS in specific-pathogen-free (SPF) mice. Fecal samples of 40 mouse lines were analyzed for members of the family Helicobacteraceae using a group-specific PCR targeting the 16S rRNA gene. Additional experiments were carried out to evaluate the spread of EHS among mice harbored in different caging systems. Helicobacter species were detected in 87.5% of the mouse lines tested. Five different Helicobacter species were identified: H. ganmani, H. hepaticus, H. typhlonicus, and the putative Helicobacter species represented by the isolates hamster B and MIT 98-5357. Helicobacter infection did not spread between animals in neighboring cages when individually ventilated cages were used; in contrast, when the mice were reared in open-air cages, EHS were found to spread from cage to cage. However, the spread was prevented by adding polycarbonate filter tops to the cages. When Helicobacter-negative and infected mice shared the same cage, transmission of the infection...Continue Reading

References

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Jul 1, 1997·International Journal of Systematic Bacteriology·Z ShenL Perry
May 2, 2000·Journal of Clinical Microbiology·J H SimmonsC L Franklin
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Feb 8, 2002·Laboratory Animals·W NicklasUNKNOWN FELASA (Federation of European Laboratory Animal Science Associations Working Group on Health Monitoring of Rodent and Rabbi
Dec 18, 2002·Helicobacter·Ulrich R M BohrPeter Malfertheiner
Jun 9, 2004·Journal of Clinical Microbiology·Ulrich R M BohrPeter Malfertheiner

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Citations

Feb 2, 2012·Inflammatory Bowel Diseases·Trenton R Schoeb, Daniel C Bullard
Sep 11, 2012·Applied and Environmental Microbiology· WasimuddinJaroslav Piálek
Jun 16, 2010·International Journal of Radiation Biology·Thomas TillmannClemens Dasenbrock
Oct 22, 2011·Pathology, Research and Practice·Sabine KruegerDoerthe Kuester
Oct 11, 2007·Helicobacter·Ulrich R M BohrAntonio Gasbarrini
Aug 24, 2006·Helicobacter·Jay V SolnickAntonio Gasbarrini
Mar 5, 2015·International Journal of Systematic and Evolutionary Microbiology·Shoukui HuJianguo Xu
Mar 10, 2007·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·U R M BohrP Malfertheiner
Jan 28, 2018·The Journal of Experimental Medicine·Jason MiskaZhibin Chen
Feb 23, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·David C JayPeter E Jensen
Oct 23, 2018·Experimental and Therapeutic Medicine·Jianjun RenClaus Bachert
Apr 29, 2014·Experimental Animals·Mathurot DuangchanchotKanchana Kengkoom
Sep 7, 2018·Neuropsychopharmacology : Official Publication of the American College of Neuropsychopharmacology·Amanda C KentnerMelissa D Bauman
Mar 6, 2009·Gastroenterology·Makoto Mark Taketo, Winfried Edelmann

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