Prevalence characterization of extended-spectrum beta-lactamases among Escherichia coli isolates collected in Zhengzhou.

Journal of Clinical Laboratory Analysis
Zhijian ZhangQinxian Zhang

Abstract

To determine the prevalence and the diversity of extended-spectrum beta-lactamases (ESBLs) among Escherichia coli isolates in Zhengzhou, China. Clinical isolates were collected and investigated from the first affiliated hospital of Zhengzhou University and its associated health-care facilities in Zhengzhou, China, during the period from January 2006 to June 2008. Antibiograms were performed on Mueller-Hinton agar plates with the disc-diffusion method and MICs were determined by the agar-dilution method. Total DNA was extracted with a Qiagen mini kit and screened by PCR. Of 94 nonduplicate ESBL-positive isolates, TEM-type was encoded in 74 and 79% of the ESBL isolates. Fifty-six isolates were SHV type ESBLs. CTX-M-1, CTX-M-14, CTX-M-25, and CTX-M-38 types were encoded in 30, 54, 6, and 4, respectively. OXA-1-type beta-lactamases were encoded in six and OXA-20-type was encoded in two isolates. We describe a complex ESBL epidemiology. The study revealed a high rate of ESBL-producing E. coli isolates. TEM and CTX-M enzymes dominated in ESBL-positive E. coli isolates in Zhengzhou, China.

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