Prevalence estimation and risk factors for Escherichia coli O157 on Dutch dairy farms

Preventive Veterinary Medicine
J M SchoutenE A M Graat

Abstract

To estimate the prevalence of Escherichia coli O157 on Dutch dairy herds, faecal samples were collected once from 678 randomly selected dairy farms in the period October 1996-December 2000. Samples were cultured for E. coli O157. Thirty-eight isolates were tested for virulence genes (eae, VT1 and VT2). A questionnaire about farm characteristics was taken from the farm manager, resulting in variables that could be analysed to identify and quantify factors associated with presence of E. coli O157. In total, 49 of the 678 herds (7.2%) showed at least one positive pooled sample. E. coli O157 was not isolated from herds sampled in December-April in consecutive years (except for one isolate found in March, 2000). VT- and eae-genes were found in 37 and 38 isolates, respectively. Logistic regression was performed on variables obtained from the questionnaire, comparing E. coli O157-positive herds to negative herds. To account for season, a sine function was included in the logistic regression as an offset variable. In the final model, the presence of at least one pig at the farm (OR = 3.4), purchase of animals within the last 2 years before sampling (OR = 1.9), supply of maize (OR = 0.29) to the cows, and sampling a herd in the year 199...Continue Reading

References

Feb 1, 1997·Epidemiology and Infection·S C MechieC A Siddons
Apr 1, 1997·Epidemiology and Infection·D D HancockP I Tarr
Nov 26, 1999·International Journal of Food Microbiology·A E HeuvelinkE de Boer
Aug 13, 2002·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·Y T H P Van DuynhovenW J B Wannet
Sep 12, 2002·Veterinary Microbiology·S L BooherH W Moon

❮ Previous
Next ❯

Citations

May 17, 2013·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·O PinakaC Hadjichristodoulou
Feb 23, 2007·Epidemiology and Infection·A E HeuvelinkE De Boer
Nov 15, 2008·Nature Reviews. Microbiology·Margo Chase-ToppingMark Woolhouse
Dec 2, 2010·Foodborne Pathogens and Disease·Witold A Ferens, Carolyn J Hovde
Apr 10, 2013·Foodborne Pathogens and Disease·Maria-Adelheid JorisLieven De Zutter
Mar 16, 2007·Journal of Clinical Microbiology·Margo E Chase-ToppingMark E J Woolhouse
Apr 2, 2014·Frontiers in Microbiology·Leonard S van OverbeekPeter T J Willemsen
Jun 22, 2007·Preventive Veterinary Medicine·Johanne Ellis-IversenGiles A Paiba
Jan 10, 2008·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Mark E ArnoldGeoff C Pritchard
Aug 25, 2006·Nature·Hiroshi OhmotoBruce E Taylor
Aug 30, 2008·Critical Reviews in Microbiology·Eelco Franz, Ariena H C van Bruggen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Antibodies: Agglutination

Antibody-mediated agglutination is the clumping of cells in the presence of antibody, which binds multiple cells together. This enhances the clearance of pathogens. Find the latest research on antibody-mediated agglutination here.