Prevalence, molecular epidemiology, and antimicrobial resistance of methicillin-resistant Staphylococcus aureus from swine in southern Italy

BMC Microbiology
Mattia PiroloPaolo Visca

Abstract

Colonization by livestock-associated MRSA (LA-MRSA) has increasingly been reported in the swine population worldwide. The aim of this study was to assess the prevalence of MRSA nasal carriage in healthy pigs, including the black (Calabrese) breed, from farms in the Calabria Region (Southern Italy). Between January and March 2018, a total of 475 healthy pigs reared in 32 farms were sampled by nasal swabbing. MRSA isolates were characterized by spa, MLST and SCCmec typing, and susceptibility testing to 17 antimicrobials. 22 of 32 (66.8%) pig farms resulted positive for MRSA. The prevalence of MRSA was 46.1% (219 MRSA culture-positive out of 475 samples). MRSA colonization was significantly higher in intensive farms and in pigs with a recent or ongoing antimicrobial treatment. All 219 MRSA isolates were assigned to ST398. The most common spa types were t011 (37.0%), t034 (22.4%) and t899 (15.1%). A novel spa type (t18290) was detected in one isolate. An insertion of IS256 in the ST398-specific A07 fragment of the SAPIG2195 gene was detected in 10 out of 81 t011 isolates. Nearly all isolates carried the SCCmec type V element, except 11 isolates that carried the SCCmec type IVc. None of the isolates was positive for the Panton-Valen...Continue Reading

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Citations

Dec 11, 2019·International Journal of Veterinary Science and Medicine·Khaled A Abdel-Moein, Hala M Zaher
Feb 12, 2021·Clinical Microbiology Reviews·Soyoun Park, Jennifer Ronholm
Jan 6, 2021·Applied and Environmental Microbiology·Henok Ayalew TegegneRenáta Karpíšková
Aug 11, 2020·International Journal of Food Microbiology·Paola TomaoPaolo Visca

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Methods Mentioned

BETA
PCR
genotyping
PCRs

Software Mentioned

Sigma Plot
Systat
BioNumerics

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