Prevalence of Acquired Carbapenemase Genes in Klebsiella Pneumoniae by Multiplex PCR in Isfahan

Advanced Biomedical Research
Farzin KhorvashNirvana Tavahen

Abstract

Multi-drug resistant Klebsiella pneumoniae has been considered as a serious global threat. This study was done to investigate carbapenemase producing genomes among K. pneumoniae isolates in Isfahan, Central Iran. In a cross-sectional study from 2011 to 2012, 29 carbapenem resistant (according to disc diffusion method) carbapenemase producing (according to modified Hodge test) K. pneumoniae strains were collected from Intensive Care Unit (ICUs) of Al-Zahra referral Hospital. In the strains with the lack of sensitivity to one or several carbapenems, beta-lactams, or beta-lactamases, there has been performed modified Hodge test to investigate carbapenmase and then only strains producing carbapenmases were selected for molecular methods. In this study, there have been 29 cases of K. pneumoniae isolated from hospitalized patients in the (ICU). Three cases (10.3%) contained blaVIM, 1 case (3.4%) contained blaIMP, and 1 case (3.4%) contained blaOXA. The genes blaNDM and blaKPC were not detected. Then, 16 cases (55.2%) from positive cases of K. pneumoniae were related to the chip, 4 cases (13.8%) to catheter, 6 cases (20.7%) to urine, and 3 cases (10.3%) to wound. It is necessary to monitor the epidemiologic changes of these carbapenem...Continue Reading

Citations

Aug 17, 2018·Acta Microbiologica Et Immunologica Hungarica·Mehdi MoghadampourJamshid Faghri
Apr 30, 2020·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mohammad Javad NasiriPayam Tabarsi
Jul 20, 2018·Acta Microbiologica Et Immunologica Hungarica·Mehdi MoghadampourJamshid Faghri

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Methods Mentioned

BETA
PCR
electrophoresis

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