Prevalence of an ulcerative colitis-associated CD8+ T cell receptor beta-chain CDR3-region motif and its association with disease activity

Journal of Clinical Immunology
C S ProbertR S Blumberg

Abstract

The normal human intestinal mucosa contains clonal T cell expansions. Clonal populations of T cells can be determined through evaluation of the idiotypic, hypervariable region of their T cell receptor (TCR). We have previously reported that there exists a highly conserved TCR pattern among intestinal CD8+ T cells in the majority of ulcerative colitis (UC) patients undergoing colectomy that was not present in normal control individuals. This TCR pattern, or motif, was characterized by particular beta-chain usage (TCRBV3 and TCRBJ1S6) and a defined length in the hypervariable third complementarity determining region (CDR3). The aim of this study was to assess the motif's relationship to disease activity. Subjects were 66 with UC, 19 with Crohn's disease, 14 inflammatory controls, and 6 normal controls. cDNA and gDNA were prepared from colonic biopsies and paraffin blocks, respectively, obtained from study subjects and used to assess TCRBV CDR3 region length and usage, as well as for cloning and sequencing of TCRs. The TCRBV CDR3 region was present in 25 of a series of 48 UC subjects but only 3 of 19 Crohn's disease patients and 3 of 14 inflammatory controls. The motif was more common in UC than either Crohn' s disease or inflamma...Continue Reading

Citations

Feb 13, 2007·Immunological Reviews·Christopher S J ProbertRichard S Blumberg
Sep 29, 2011·The Journal of Clinical Investigation·James C LeeKenneth G C Smith
Jun 28, 2005·American Journal of Human Biology : the Official Journal of the Human Biology Council·Tabita HünemeierJosé A B Chies
Feb 8, 2020·Genes and Immunity·Keerthana SaravanarajanShigeki Nakagome
Feb 9, 2011·Immunology and Cell Biology·John J MilesDavid A Price
May 13, 2017·Inflammatory Bowel Diseases·Sezin GünaltayElisabeth Hultgren Hörnquist

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