Prevalence of antimicrobial resistance genes in Bacteroides spp. and Prevotella spp. Dutch clinical isolates

Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases
A C M VelooJ W Rossen

Abstract

The prevalence of resistance genes in two important anaerobic genera, Bacteroides and Prevotella, was assessed by applying PCR specifically directed to genes of interest. A total of 101 Bacteroides spp. and 99 Prevotella spp. human clinical isolates were identified using MALDI-TOF MS. The presence of the resistance genes cfxA, cepA, cfiA, tetQ, ermF and nim, was assessed. Prevalence of resistance genes was compared with the phenotypic resistance against amoxicillin, clindamycin, meropenem and metronidazole. Even though the majority of the Bacteroides isolates (95.0%) showed resistance towards amoxicillin, only 52/101 of the isolates harboured one of the resistance genes, accounting for this resistance. Within the genus Prevotella the presence of cfxA (50/99) almost perfectly matched the amoxicillin resistance (48/99). No difference in prevalence of the ermF gene (16/101 and 9/99) and clindamycin resistance (16/101 and 10/99) was observed within Bacteroides and Prevotella, respectively. Two isolates of Prevotella were resistant to metronidazole. One harboured the nim gene. One metronidazole-susceptible isolate of Bacteroides harboured a nim gene. Within the Bacteroides and Prevotella genera, 6/101 strains and 5/99 isolates harbo...Continue Reading

Citations

Oct 1, 2019·Current Opinion in Infectious Diseases·Louise Cooley, Jasmine Teng
Nov 13, 2019·Journal of Bacteriology·Natasha A Bencivenga-BarryAndrew L Goodman
Sep 10, 2020·BMJ Case Reports·Abuzar Ali AsifSharjeel Ahmad
Oct 10, 2020·Environmental Science : Water Research & Technology·William R Morales MedinaN L Fahrenfeld
Nov 1, 2020·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Paul-Louis WoertherEtienne Ruppé
Jan 29, 2021·Journal of Global Antimicrobial Resistance·Sebastian Martin Michael RongCatalina-Suzana Stingu
Feb 10, 2021·Scientific Reports·Helena JuricovaIvan Rychlik
Sep 21, 2020·Microbial Pathogenesis·Mina YekaniMohammad Yousef Memar
Feb 7, 2021·Applied and Environmental Microbiology·Paiboon TunsagoolGunnaporn Suriyaphol
Jul 6, 2021·JAC-antimicrobial Resistance·Anshul SoodArchana Angrup
Oct 9, 2021·NPJ Biofilms and Microbiomes·Vishnu Prasoodanan P KVineet K Sharma

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