Prevalence of carbapenem-resistant Enterobacteriaceae and emergence of high rectal colonization rates of blaOXA-181-positive isolates in patients admitted to two major hospital intensive care units in Kuwait

PloS One
Amani H Al FadhliVincent O Rotimi

Abstract

Fecal colonization by carbapenem-resistant Enterobacteriaceae (CRE) can be the main reservoir for transmission of these resistant organisms especially in the Intensive Care Units (ICUs). This study was conducted to evaluate the rate of rectal carriage and molecular characterization of CRE in patients hospitalized in the ICUs of 2 major hospitals (Adan and Mubarak Al Kabeer Hospitals) in Kuwait. Rectal swabs were collected from all patients at admission, 48 h after admission and once weekly from April 2017- March 2018. Initial CRE screening was carried out on MacConkey agar on which meropenem disc 10μg was placed. Identification of isolates was by API 20E. Susceptibility testing was performed using the E-test method. Polymerase chain reaction (PCR) was used to detect the carbapenemase-encoding genes. Clonal relationship was investigated by pulsed-field electrophoresis (PFGE). Genes of blaOXA-181 and blaNDM-5-carrying plasmids were detected in some strains. A total of 590 patients were recruited into the study. Of these, 58 were positive for CRE, giving a prevalence of 9.8%; 25/320 (7.8%) in Adan and 33/270 (12.2%) in Mubarak Al Kabeer Hospitals. All isolates were resistant to multiple antibiotics. Resistance rates to colistin an...Continue Reading

References

Mar 1, 1981·Journal of Bacteriology·C I Kado, S T Liu
Dec 25, 2003·Antimicrobial Agents and Chemotherapy·Laurent PoirelPatrice Nordmann
Feb 23, 2008·The Lancet Infectious Diseases·Johann D D Pitout, Kevin B Laupland
Feb 26, 2009·The Journal of Antimicrobial Chemotherapy·Pierre BogaertsYouri Glupczynski
Apr 8, 2010·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Debby Ben-DavidGalia Rahav
Sep 4, 2010·The Journal of Antimicrobial Chemotherapy·Laure Vidal-NavarroJean-Philippe Lavigne
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Laurent PoirelPatrice Nordmann
Jul 20, 2011·Antimicrobial Agents and Chemotherapy·Anaïs PotronLaurent Poirel
Aug 11, 2011·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Debby Ben-DavidUNKNOWN Israel PACF CRKP (Post-Acute-Care Facility Carbapenem-Resistant Klebsiella pneumoniae) Working Group
Mar 13, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·L DortetP Nordmann
Apr 14, 2012·The Journal of Antimicrobial Chemotherapy·Laurent PoirelPatrice Nordmann
Nov 2, 2012·Journal of Clinical Microbiology·A LernerY Carmeli
May 4, 2013·Journal of Medical Microbiology·Wafaa JamalLaurent Poirel
May 15, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M N D BalmJ W P Teo
Jul 11, 2013·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Mahesh SwaminathanDavid P Calfee
Aug 10, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·D GirlichP Nordmann
Apr 30, 2014·American Journal of Infection Control·Zhi-chang ZhaoBin Li
Jan 1, 2015·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Wafaa Y JamalVincent O Rotimi
Feb 7, 2015·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·P VialeR E Lewis
May 20, 2015·Emerging Infectious Diseases·Sun Young ChoJae-Hoon Song
Oct 30, 2015·Clinical Microbiology Reviews·Roberto ViauRobert A Bonomo
Nov 11, 2015·Antimicrobial Agents and Chemotherapy·Windy D TannerJames A VanDerslice
Apr 7, 2016·Emerging Microbes & Infections·Doaa GamalLuis Martínez-Martínez
Mar 28, 2017·BMC Infectious Diseases·Norihisa YamamotoKazunori Tomono
May 4, 2017·Antimicrobial Agents and Chemotherapy·Laura J RojasRobert A Bonomo
Jun 24, 2017·European Journal of Clinical Microbiology & Infectious Diseases : Official Publication of the European Society of Clinical Microbiology·H SolgiF Shahcheraghi
Jan 22, 2018·Journal of Global Antimicrobial Resistance·Ibrahim BitarSima Tokajian

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Datasets Mentioned

BETA
MK256964.1
MF774789.1

Methods Mentioned

BETA
PCR
electrophoresis

Software Mentioned

Epical 2000
SPSS Statistics

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