PMID: 12781480Jun 5, 2003Paper

Prevalence of cpb2, encoding beta2 toxin, in Clostridium perfringens field isolates: correlation of genotype with phenotype

Veterinary Microbiology
Dawn M BueschelJ Glenn Songer

Abstract

Beta2 toxin, encoded by the cpb2 gene, has been implicated in the pathogenesis of porcine, equine and bovine enteritis by type A Clostridium perfringens. By incorporating primers to cpb2 into a multiplex genotyping PCR, we screened 3270 field isolates of C. perfringens. Of these, 37.2% were PCR positive for the cpb2 gene. The majority of isolates from cases of porcine enteritis were positive for cpb2 (>85%), and this was even more true for C. perfringens isolated from cases of porcine neonatal enteritis (91.8%). In contrast, isolates from normal pigs only contained cpb2 in 11.1% of cases. The correlation between enteritis in other animal species and the presence of cpb2 was not so strong. cpb2 was found in 21.4% of C. perfringens isolates from cattle with enteritis, and in 47.3% of isolates from calves with enteritis or abomastitis. The prevalence of cpb2 varied with genotype, with type A isolates being positive for this gene in 35.1% of cases. Furthermore, enterotoxigenic type D or type E strains almost always carried cpb2. We cloned a 6xHIS-tagged beta2 (HIS-beta2) and used this protein to raise antiserum against beta2. Culture supernatants from 68 cpb2-positive and 13 cpb2-negative strains were tested for the presence of bet...Continue Reading

References

Jun 1, 1995·Trends in Genetics : TIG·A Pospiech, B Neumann
Jan 13, 1998·Gene·M GibertC Jolivet-Renaud
Jul 9, 1999·FEMS Immunology and Medical Microbiology·H L KlaasenJ F van den Bosch
Jan 17, 2002·Proceedings of the National Academy of Sciences of the United States of America·Tohru ShimizuHideo Hayashi
Mar 20, 2002·Veterinary Microbiology·C MantecaJ G Mainil

❮ Previous
Next ❯

Citations

Jun 28, 2005·Journal of the American College of Surgeons·Jeremy SobelClifford MacDonald
Mar 15, 2005·Avian Pathology : Journal of the W.V.P.A·Filip Van ImmerseelRichard Ducatelle
Aug 14, 2010·Avian Pathology : Journal of the W.V.P.A·Guangxing LiGregory R Siragusa
Aug 14, 2010·Avian Pathology : Journal of the W.V.P.A·Guangxing LiGregory R Siragusa
Dec 15, 2010·Avian Pathology : Journal of the W.V.P.A·Jessica BradyJuan C Rodriguez-Lecompte
Jun 29, 2010·Applied and Environmental Microbiology·Sabrina R Mueller-SpitzSandra L McLellan
May 24, 2011·Applied and Environmental Microbiology·Janneke G AllaartAndrea Gröne
Jan 31, 2007·Infection and Immunity·Jihong LiBruce A McClane
Feb 3, 2005·CMAJ : Canadian Medical Association Journal = Journal De L'Association Medicale Canadienne·Donald C Vinh
Jan 13, 2010·Journal of Zoo and Wildlife Medicine : Official Publication of the American Association of Zoo Veterinarians·Georgios N NikolaouAndrea Gröne
Jan 17, 2014·The Journal of Veterinary Medical Science·Ki-Eun LeeDong-Jun An
Jan 13, 2016·BMC Research Notes·Vera L A RuizPaloma O Tonietti
Dec 20, 2011·Veterinary Microbiology·F A UzalJ G Songer
Nov 16, 2006·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·L CeciG Carelli
May 17, 2006·Anaerobe·Youhanna S Sawires, J Glenn Songer
Nov 18, 2005·Veterinary Microbiology·D R ThompsonJ F Prescott
Aug 15, 2015·Journal of Medical Microbiology·Jenny LarssonMagdalena Jacobson
Jan 1, 2014·Korean Journal for Food Science of Animal Resources·Sun-Kyung HanWan-Kyu Lee
May 28, 2013·Toxicon : Official Journal of the International Society on Toxinology·Michel R Popoff, Philippe Bouvet
Jan 13, 2015·International Journal of Food Microbiology·Conor J DoylePaul D Cotter
Apr 18, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Jorge P GarciaFrancisco A Uzal
Apr 11, 2013·Journal of Veterinary Diagnostic Investigation : Official Publication of the American Association of Veterinary Laboratory Diagnosticians, Inc·Kerry K CooperFrancisco A Uzal

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.