Prevalence of Extended-Spectrum Beta-Lactamase/Carbapenemase Genes and Quinolone-Resistance Determinants in Klebsiella pneumoniae Clinical Isolates from Respiratory Infections in Myanmar.

Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease
Meiji Soe AungNobumichi Kobayashi

Abstract

In recent years, nosocomial infections due to multidrug resistant Klebsiella pneumoniae strains have been increasing, associated with growing trend of resistance to beta-lactams and fluoroquinolones (FQs) worldwide. In this study, prevalence of beta-lactamase genes and resistance mechanisms to FQ were analyzed in 191 clinical K. pneumoniae isolates derived from respiratory tract infections in a teaching hospital in Yangon, Myanmar. The major extended-spectrum beta-lactamase gene was blaCTX-M, which was detected in 33% of isolates, with CTX-M-15 being dominant. Fourteen isolates (7.3%) harbored carbapenemase genes that were genotyped as blaNDM-1, blaNDM-5, or blaNDM-7. The most common plasmid-mediated quinolone resistance (PMQR) gene was aac6'-Ib-cr (51.8%), followed by qnrB (41.9%), oqxAB (23%), and qnrS (15.2%). In quinolone-resistance determining region of GyrA, eight different types of mutation were identified for FQ-resistant isolates, with double mutations at two positions (S83F, D87A) being most common (54.6%). Isolates with double mutations (three patterns) showed higher minimal inhibitory concentration to levofloxacin (LVX) (≥64 μg/mL) than those with a single mutation. PMQR gene profiles, including aac6'-Ib-cr and any ...Continue Reading

References

May 31, 2002·Journal of Clinical Microbiology·F Javier Pérez-Pérez, Nancy D Hanson
Apr 19, 2003·The Journal of Antimicrobial Chemotherapy·A M Emmerson, A M Jones
Dec 8, 2004·Current Drug Targets. Infectious Disorders·Yun-Liang YangHsiu-Jung Lo
Aug 6, 2005·Journal of Clinical Microbiology·Laure DiancourtSylvain Brisse
Nov 10, 2005·Journal of Medical Microbiology·Li XuPeter Hawkey
Jul 17, 2007·Clinical Microbiology Reviews·Anne Marie Queenan, Karen Bush
Jan 11, 2008·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·H-J MonsteinL E Nilsson
Dec 10, 2008·Antimicrobial Agents and Chemotherapy·Hong Bin KimDavid C Hooper
Mar 12, 2009·Current Medicinal Chemistry·Vincent Cattoir, Patrice Nordmann
Jun 6, 2009·Infection Control and Hospital Epidemiology : the Official Journal of the Society of Hospital Epidemiologists of America·Khetam HusseinRenato Finkelstein
Aug 15, 2009·Drugs·Xian-Zhi Li, Hiroshi Nikaido
Oct 14, 2009·Clinical Microbiology Reviews·Jacob StrahilevitzAri Robicsek
Feb 26, 2010·Journal of Clinical Microbiology·Kenneth S Thomson
Mar 15, 2011·Diagnostic Microbiology and Infectious Disease·Laurent PoirelPatrice Nordmann
Aug 3, 2012·The Journal of Antimicrobial Chemotherapy·Monica ÖsterbladJari Jalava
Mar 5, 2013·Diagnostic Microbiology and Infectious Disease·Longhua HuJingye Pan
May 3, 2014·BioMed Research International·Laurent DortetPatrice Nordmann
May 21, 2014·Trends in Microbiology·Liam S RedgraveLaura J V Piddock
Jan 15, 2015·Microbiology Spectrum·George A JacobyDavid C Hooper
Jul 15, 2015·Antimicrobial Agents and Chemotherapy·Johann D D PitoutLaurent Poirel
May 12, 2016·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Guanhua XueHongmei Sun
May 19, 2017·FEMS Microbiology Reviews·Shiri Navon-VeneziaAlessandra Carattoli
May 26, 2017·The Journal of Antimicrobial Chemotherapy·Edward R BevanPeter M Hawkey
Mar 23, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Meiji Soe AungNobumichi Kobayashi
Apr 8, 2018·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Yong ChongNobuyuki Shimono
Nov 21, 2018·Microbial Drug Resistance : MDR : Mechanisms, Epidemiology, and Disease·Mona H Abdel-RahimSherine A Aly

❮ Previous
Next ❯

Datasets Mentioned

BETA
MN786376
MN786391

Methods Mentioned

BETA
PCR
genotyping

Software Mentioned

MEGAX
BLAST ( Basic Local Alignment Search Tool )

Related Concepts

Related Feeds

CRISPR Screens in Drug Resistance

CRISPR-Cas system enables the editing of genes to create or correct mutations. This feed focuses on the application of CRISPR-Cas system in high-throughput genome-wide screens to identify genes that may confer drug resistance.

© 2022 Meta ULC. All rights reserved