Prevalence of Fosfomycin Resistance and Mutations in murA, glpT, and uhpT in Methicillin-Resistant Staphylococcus aureus Strains Isolated from Blood and Cerebrospinal Fluid Samples

Frontiers in Microbiology
Zhuyingjie FuMinggui Wang

Abstract

In China, fosfomycin alone or in combination with other antibiotics is commonly used in the treatment of infections caused by Gram-positive bacteria, including methicillin-resistant Staphylococcus aureus (MRSA). Although fosfomycin-resistant S. aureus strains have continued to emerge and increase, the research on them is rare. In order to determine the prevalence and mechanisms of fosfomycin resistance in MRSA clinical isolates, a total of 96 non-duplicate MRSA isolates were collected from blood and cerebrospinal fluid samples at Huashan Hospital in Shanghai, China between 2004 and 2014. Antimicrobial susceptibility testing was performed by agar dilution. Meanwhile, the fosfomycin-resistance-related genes, fosB, murA, glpT, and uhpT, were amplified by PCR and subjected to sequencing analysis. Multilocus sequence typing (MLST) was conducted to assess strain types. The minimum inhibitory concentration (MIC) of fosfomycin for the 96 MRSA strains ranged from 1.0 to >1,024 mg/L, and approximately 70% (67/96) of the isolates were resistant to fosfomycin (MIC ≥ 64.0 mg/L). Nine isolates with MICs ≥ 128 mg/L carried fosB gene. Twenty-five distinct mutations were detected in the murA (7), glpT (10), and uhpT (8) genes. While five of the...Continue Reading

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Citations

Oct 13, 2018·Drug Development Research·Marko JukičMatej Sova
Nov 16, 2018·Journal of Medical Microbiology·Mina AghamaliEbrahim Kouhsari
Apr 23, 2019·Omics : a Journal of Integrative Biology·Relangi Tulasi RaoKannan Jayakumar
Aug 19, 2020·Antimicrobial Resistance and Infection Control·Yi-Chien LeeShan-Chwen Chang
Dec 21, 2017·Frontiers in Microbiology·Axel CloeckaertBenoît Doublet
Mar 23, 2019·Microbiology Spectrum·Martin VestergaardHanne Ingmer

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Methods Mentioned

BETA
PCR

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